Team:Chiba/Parts/test

From 2010.igem.org

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(Hybrid promoter)
(Reporters)
 
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
 
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[[Image:Chiba-U.gif|center]]
 
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
 
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!align="center"|[[Team:Chiba|Home]]
 
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!align="center"|[[Team:Chiba/Team|The Team]]
 
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!align="center"|[[Team:Chiba/Project|The Project]]
 
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!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Chiba/Reference|Reference]]
 
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
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|}
 
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{| align="justify"
 
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|
 
==Parts Status(Parts created by Chiba)==
==Parts Status(Parts created by Chiba)==
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
 
-
!align="center"|[[Team:Chiba|Home]]
 
-
!align="center"|[[Team:Chiba/Team|The Team]]
 
-
!align="center"|[[Team:Chiba/Project|The Project]]
 
-
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:Chiba/Reference|Reference]]
 
-
!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
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|}
 
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===Hybrid promoter===
===Hybrid promoter===
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<td width="200">Sequenced<td>
<td width="200">Sequenced<td>
<td width="200">Sent<td>
<td width="200">Sent<td>
-
<tr>
+
</tr><tr>
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<tr>
+
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td>
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td>
<td width="300">pLux/cI434 Hybrid promoter</td>
<td width="300">pLux/cI434 Hybrid promoter</td>
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</tr>
</tr>
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===Reporters===
+
===Reporter===
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center>
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center>
<tr>
<tr>
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<td width="200">Sequenced<td>
<td width="200">Sequenced<td>
<td width="200">Sent<td>
<td width="200">Sent<td>
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</tr>
</tr>
<tr>
<tr>
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<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
-
 
</tr>
</tr>
<tr>
<tr>
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<td width="200">2886<td>
<td width="200">2886<td>
<td width="200">Cm<td>
<td width="200">Cm<td>
-
<td width="350">80/miniprepped<td>
+
<td width="350">miniprepped<td>
<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
</tr>
</tr>
-
 
+
===Composite===
-
==Parts Status(Parts used by Chiba)==
+
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center>
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center>
<tr>
<tr>
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<td width="200">Status<td>
<td width="200">Status<td>
<td width="200">Sequenced<td>
<td width="200">Sequenced<td>
 +
<td width="200">Sent<td>
</tr>
</tr>
<tr>
<tr>
-
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td>
+
<td width="200">[http://partsregistry.org/Part:BBa_K396000 BBa_K396000]</td>
-
<td width="300">Promoter (HSL-mediated luxR repressor)</td>
+
<td width="300">[http://partsregistry.org/Part:BBa_K396000 T7/CI Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396011 (composite with LuxR generator)]</td>
-
<td width="200">30<td>
+
<td width="200">806<td>
-
<td width="200">pSB2K3<td>
+
<td width="200">pSB1C3<td>
-
<td width="200">F and P1 lytic<td>
+
<td width="200">pMB1<td>
-
<td width="200">4425<td>
+
<td width="200">2072<td>
-
<td width="200">4455<td>
+
<td width="200">2878<td>
-
<td width="200">Kan<td>
+
<td width="200">Cm<td>
<td width="350">miniprepped<td>
<td width="350">miniprepped<td>
-
<td width="50">OK<td>
+
<td width="50">ok<td>
-
</tr>
+
<td width="50">ok<td>
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td>
+
-
<td width="300">ptet+cI</td>
+
-
<td width="200">992<td>
+
-
<td width="200">pSB1A2<td>
+
-
<td width="200">pMB1<td>
+
-
<td width="200">2079<td>
+
-
<td width="200">3071<td>
+
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<td width="200">Amp<td>
+
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<td width="350">Glycerol stock in -80/miniprepped<td>
+
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<td width="50">OK<td>
+
</tr>
</tr>
-
===Receiver protein===
+
===Device===
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
+
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center>
<tr>
<tr>
<td width="200">Part Name</td>
<td width="200">Part Name</td>
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</tr>
</tr>
<tr>
<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td>
+
<td width="200">[http://partsregistry.org/Part:BBa_K396006 BBa_K396006]</td>
-
<td width="300">ptet+rbs+RhlR</td>
+
<td width="300">[http://partsregistry.org/Part:BBa_K396001 pLux/cI434 Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396012 cI434 generator]</td>
-
<td width="200">867<td>
+
<td width="200">1654<td>
-
<td width="200">pSB1A2<td>
+
<td width="200">pSB1C3<td>
<td width="200">pMB1<td>
<td width="200">pMB1<td>
-
<td width="200">2079<td>
+
<td width="200">2072<td>
-
<td width="200">2946<td>
+
<td width="200">3726<td>
-
<td width="200">Amp<td>
+
<td width="200">Cm<td>
<td width="350">miniprepped<td>
<td width="350">miniprepped<td>
<td width="50">ok<td>
<td width="50">ok<td>
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
<tr>
 
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<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td>
 
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<td width="300">ptet+rbs+RhlR</td>
 
-
<td width="200">867<td>
 
-
<td width="200">pSB3K3<td>
 
-
<td width="200">p15A<td>
 
-
<td width="200">2750<td>
 
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<td width="200">3617<td>
 
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<td width="200">Kan<td>
 
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<td width="350">miniprepped<td>
 
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<td width="50">Not yet<td>
 
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<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
<tr>
 
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<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td>
 
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<td width="300">ptet+rbs+LasR</td>
 
-
<td width="200">861<td>
 
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<td width="200">pSB1A2<td>
 
-
<td width="200">pMB1<td>
 
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<td width="200">2079<td>
 
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<td width="200">2940<td>
 
-
<td width="200">Amp<td>
 
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<td width="350">miniprepped<td>
 
<td width="50">ok<td>
<td width="50">ok<td>
-
<td width="50">[[Image:Chiba check.png]]<td>
 
</tr>
</tr>
<tr>
<tr>
-
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td>
+
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 BBa_K396007]</td>
-
<td width="300">ptet+rbs+LasR</td>
+
<td width="300">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 T7 RNA Polymerase pulse generator]</td>
-
<td width="200">861<td>
+
<td width="200">3542<td>
-
<td width="200">pSB3K3<td>
+
<td width="200">pSB1C3<td>
-
<td width="200">p15A<td>
+
-
<td width="200">2750<td>
+
-
<td width="200">3611<td>
+
-
<td width="200">Kan<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">Not yet<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td>
+
-
<td width="300">ptet+rbs+CinR</td>
+
-
<td width="200">867<td>
+
-
<td width="200">pSB1A2<td>
+
<td width="200">pMB1<td>
<td width="200">pMB1<td>
-
<td width="200">2079<td>
+
<td width="200">2072<td>
-
<td width="200">2946<td>
+
<td width="200">5614<td>
-
<td width="200">Amp<td>
+
<td width="200">Cm<td>
<td width="350">miniprepped<td>
<td width="350">miniprepped<td>
<td width="50">ok<td>
<td width="50">ok<td>
-
<td width="50">[[Image:Chiba check.png]]<td>
+
<td width="50">ok<td>
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td>
+
-
<td width="300">ptet+rbs+CinR</td>
+
-
<td width="200">867<td>
+
-
<td width="200">pSB3K3<td>
+
-
<td width="200">p15A<td>
+
-
<td width="200">2750<td>
+
-
<td width="200">3617<td>
+
-
<td width="200">Kan<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">Not yet<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
</tr>
</tr>
-
</table>
 
-
===Reporters===
+
==Parts Status(Parts used by Chiba)==
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
+
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#33FF99" center>
<tr>
<tr>
<td width="200">Part Name</td>
<td width="200">Part Name</td>
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<td width="200">Status<td>
<td width="200">Status<td>
<td width="200">Sequenced<td>
<td width="200">Sequenced<td>
-
<td width="200">Sent<td>
 
</tr>
</tr>
<tr>
<tr>
-
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084002 BBa_K084002]</td>
+
<td width="200">[http://partsregistry.org/Part:BBa_R0061 BBa_R0061]</td>
-
<td width="300">rbs+venusYFP(yeast optimized)</td>
+
<td width="300">Promoter (HSL-mediated luxR repressor)</td>
-
<td width="200">767<td>
+
<td width="200">30<td>
-
<td width="200">pSB1A2<td>
+
<td width="200">pSB2K3<td>
-
<td width="200">pMB1 <td>
+
<td width="200">F and P1 lytic<td>
-
<td width="200">2079<td>
+
<td width="200">4425<td>
-
<td width="200">3915<td>
+
<td width="200">4455<td>
-
<td width="200">Amp<td>
+
<td width="200">Kan<td>
<td width="350">miniprepped<td>
<td width="350">miniprepped<td>
-
<td width="50">no<td>
+
<td width="50">OK<td>
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<td width="50">no<td>
+
</tr>
</tr>
<tr>
<tr>
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<td width="200">[http://partsregistry.org/Part:BBa_K084003 BBa_K084003]</td>
+
<td width="200">[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]</td>
-
<td width="300">ptet+LuxR+plux+venusYFP(yeast optimized)</td>
+
<td width="300">ptet+cI</td>
-
<td width="200">1836<td>
+
<td width="200">992<td>
<td width="200">pSB1A2<td>
<td width="200">pSB1A2<td>
<td width="200">pMB1<td>
<td width="200">pMB1<td>
<td width="200">2079<td>
<td width="200">2079<td>
-
<td width="200">3915<td>
+
<td width="200">3071<td>
-
<td width="200">Amp<td>
+
-
<td width="350">miniprepped<td>
+
-
<td width="50">OK<td>
+
-
<td width="50">[[Image:Chiba check.png]]<td>
+
-
</tr>
+
-
</table>
+
-
==Parts Status(Parts used by Chiba)==
+
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
+
-
<tr>
+
-
<td width="200">Part Name</td>
+
-
<td width="200">Sub Parts</td>
+
-
<td width="200">Part Length(bp)<td>
+
-
<td width="200">Plasmid Vector<td>
+
-
<td width="200">Origin<td>
+
-
<td width="300">Part Length(bp)<td>
+
-
<td width="200">Part and vector length(bp)<td>
+
-
<td width="200">Resistance<td>
+
-
<td width="200">Status<td>
+
-
<td width="200">Sequenced<td>
+
-
</tr>
+
-
<tr>
+
-
<td width="200">[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]</td>
+
-
<td width="300">ptet+LuxR+plux+GFP</td>
+
-
<td width="200">1945<td>
+
-
<td width="200">pSB1A3<td>
+
-
<td width="200">pMB1<td>
+
-
<td width="200">2257<td>
+
-
<td width="200">4102<td>
+
<td width="200">Amp<td>
<td width="200">Amp<td>
<td width="350">Glycerol stock in -80/miniprepped<td>
<td width="350">Glycerol stock in -80/miniprepped<td>
<td width="50">OK<td>
<td width="50">OK<td>
</tr>
</tr>
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]</td>
 
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<td width="300">ptet+LuxI</td>
 
-
<td width="200">723<td>
 
-
<td width="200">pSB1AC3<td>
 
-
<td width="200">pMB1<td>
 
-
<td width="200">3005<td>
 
-
<td width="200">3778<td>
 
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<td width="200">Amp/Cm<td>
 
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<td width="350">Glycerol stock in -80/miniprepped<td>
 
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<td width="50">OK<td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
 
-
!align="center"|[[Team:Chiba|Home]]
 
-
!align="center"|[[Team:Chiba/Team|The Team]]
 
-
!align="center"|[[Team:Chiba/Project|The Project]]
 
-
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:Chiba/Reference|Reference]]
 
-
!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
-
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
-
|}
 

Latest revision as of 19:09, 26 October 2010

Contents

Parts Status(Parts created by Chiba)

Hybrid promoter

Reporter

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002] pLux/cI434 Hybrid promoter 61 pSB1C3 pMB1 2072 2133 Cm miniprepped ok no

Generator

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 BBa_K396003] UV light GFP with LVA tag 723 pSB1C3 pMB1 2072 2795 Cm miniprepped ok no

Composite

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
[http://partsregistry.org/Part:BBa_K396001 BBa_K396001] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 pLux/cI434 hybrid promoter]+[http://partsregistry.org/Part:BBa_B0031 RBS weak]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396003 GFPuv LVA tag] 806 pSB1C3 pMB1 2072 2878 Cm miniprepped ok ok
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 BBa_K396011] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K396011 Prom-luxR] 814 pSB1C3 pMB1 2072 2886 Cm miniprepped ok ok

Device

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
[http://partsregistry.org/Part:BBa_K396000 BBa_K396000] [http://partsregistry.org/Part:BBa_K396000 T7/CI Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396011 (composite with LuxR generator)] 806 pSB1C3 pMB1 2072 2878 Cm miniprepped ok ok

Parts Status(Parts used by Chiba)

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
[http://partsregistry.org/Part:BBa_K396006 BBa_K396006] [http://partsregistry.org/Part:BBa_K396001 pLux/cI434 Hybrid Promoter with GFP ]+[http://partsregistry.org/Part:BBa_K396012 cI434 generator] 1654 pSB1C3 pMB1 2072 3726 Cm miniprepped ok ok
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 BBa_K396007] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K396007 T7 RNA Polymerase pulse generator] 3542 pSB1C3 pMB1 2072 5614 Cm miniprepped ok ok
Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced
[http://partsregistry.org/Part:BBa_R0061 BBa_R0061] Promoter (HSL-mediated luxR repressor) 30 pSB2K3 F and P1 lytic 4425 4455 Kan miniprepped OK
[http://partsregistry.org/Part:BBa_J01101 BBa_J01101] ptet+cI 992 pSB1A2 pMB1 2079 3071 Amp Glycerol stock in -80/miniprepped OK