Team:Chiba/Modeling
From 2010.igem.org
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Second,there is a cI promoter above gfp DNA sequence.<br> | Second,there is a cI promoter above gfp DNA sequence.<br> | ||
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cI works as repressor.<br> | cI works as repressor.<br> | ||
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You can see cI DNA sequence is under lux promoter.<br> | You can see cI DNA sequence is under lux promoter.<br> | ||
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As AHL come into Double Click Bacteria, generation of cI is stopped.<br> | As AHL come into Double Click Bacteria, generation of cI is stopped.<br> | ||
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Therefore, repressor of cI promoter disappears.<br> | Therefore, repressor of cI promoter disappears.<br> | ||
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(Because the cause of repress(cI) which is kind of protein will be decomposed.)<br> | (Because the cause of repress(cI) which is kind of protein will be decomposed.)<br> | ||
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If there isn't cI, bacteria shine with gfp.<br> | If there isn't cI, bacteria shine with gfp.<br> | ||
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- | We can say <b><font color="red">cI recognizes the existence of input in this DNA sequence.</font></b><br> | + | We can say <b><font color="red">cI recognizes the existence of input in this DNA sequence.</font></b> |
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Revision as of 07:47, 13 September 2010
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First, we use AHL for input and regard injection of AHL as clicking. |
Second,there is a cI promoter above gfp DNA sequence. |
Third, there is AND Gate with T7ptag and supD.
As you know, T7ptag becomes T7 polymerase in case of existing supD.
T7 polymerase is translated to T7 protein and T7 works as activator of T7 promoter.
There is a T7 promoter above gfp DNA sequence.
If T7 have been generated once,
the T7 promoter starts translating and we can see bacteria shine with gfp.
On initial state(there isn't any click), bacteria don't have supD.
So there isn't any T7 polymerase and gfp cannot be genernated, in spite of being T7ptag in bacteria.
T7ptag which is a kind of mRNA is decomposed so fast.
However supD under lacI promoter is generated late.
Because, transcription of supD starts working after most of lacI are decomposed.
After 1st click, supD exist.(supD is a kind of tRNA which is stable.)
There isn't input anymore, promoter above T7ptag DNA sequence starts activating.
As a result of reacting T7ptag and supD, the AND Gate is going to be available.
In this case, the AND Gate remembers that there was a click.
Moreover,
Actually, there is a hybrid promoter(cI/T7) above gfp DNA sequence.
T7 works as activator and cI works as repressor to the promoter.