Team:Cambridge/References/ProjectBioluminescence/Luciferase

Mutant (EPIC) luciferase (P. pyralis)

• Mutant (EPIC) Luciferase (P.pyralis) Sequence
• Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color
• Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis

Bacterial Bioluminescence

• Vibrio Fischeri
• Vibrio Harveyi
• Genetics of luxCDABE (adapted from wikipedia)
• 2006 attempts at BioBricking the lux operon
• Notes
• The lux wiki from parts registry

• NCBI Lux operon sequence
• parts registry lux operon -currently waiting on an email to see how useful/available this part is
• IMPORTANT INFO

Plasmid experiment

• Vibrio plasmid experiment -possible source of lux operon

File:4449975.pdf

• [1] - Transformation protocol for students from basics - includes several plasmids

• Plan
  • set plasmid 555 into a BioBrick
  • replace the promoter? sensitivity tuners?