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Team:Cambridge/References/ProjectBioluminescence/Luciferase
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Mutant (EPIC) luciferase (P. pyralis)
- Mutant (EPIC) Luciferase (P.pyralis) Sequence
- Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color
- Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
Bacterial Bioluminescence
- Vibrio Fischeri
- Vibrio Harveyi
- Genetics of luxCDABE (adapted from wikipedia)
- 2006 attempts at BioBricking the lux operon
- Notes
- The lux wiki from parts registry
- The Firefly Luciferase in the registry. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
- The Firefly Luciferase in the registry. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
- NCBI Lux operon sequence
- Parts Registry Luciferase under control of Phage promoter - T7 Phage
- IMPORTANT INFO
Plasmid experiment
- Vibrio plasmid experiment -possible source of lux operon
- [1] - Transformation protocol for students from basics - includes several plasmids
- Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?
