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Team:Cambridge/References/ProjectBioluminescence/Luciferase
From 2010.igem.org
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** The plasmid selected is [http://www.partsregistry.org/Part:BBa_J13002 BBa_J13002], which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009. | ** The plasmid selected is [http://www.partsregistry.org/Part:BBa_J13002 BBa_J13002], which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009. | ||
==Wavelengths== | ==Wavelengths== | ||
| - | 548 nm http:// | + | 548 nm http://pubs.acs.org/doi/pdf/10.1021/bi7015052 <html><img src="http://www4a.wolframalpha.com/Calculate/MSP/MSP545519beai43119h6bi900003450dhf2577a40b8?MSPStoreType=image/gif&s=62&w=99&h=20"></html> |
613 nm http://www.promega.com/pnotes/85/10904_11/10904_11.pdf <html><img src="http://www4a.wolframalpha.com/Calculate/MSP/MSP52619bedhd64d9hi9240000123ei80gh721227b?MSPStoreType=image/gif&s=40&w=99&h=20"></html> | 613 nm http://www.promega.com/pnotes/85/10904_11/10904_11.pdf <html><img src="http://www4a.wolframalpha.com/Calculate/MSP/MSP52619bedhd64d9hi9240000123ei80gh721227b?MSPStoreType=image/gif&s=40&w=99&h=20"></html> | ||
{{:Team:Cambridge/Templates/footer}} | {{:Team:Cambridge/Templates/footer}} | ||
Revision as of 12:07, 28 July 2010
Luciferase
Mutant luciferase (P. pyralis)
- Mutant Enhanced Photon Initiating Complex (EPIC) Luciferase (P.pyralis) Sequence
- Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color
- Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
Other Insect luciferases
- For colours we could use the click beetle luciferase red or green. They're reliable and from the Knight lab but not in the distribution.
Bacterial Bioluminescence
- Vibrio Fischeri
- Vibrio Harveyi
- Genetics of luxCDABE (adapted from wikipedia)
- 2006 attempts at BioBricking the lux operon
- Notes
- The lux wiki from parts registry
- NCBI Lux operon sequence
- parts registry lux operon -currently waiting on an email to see how useful/available this part is
- IMPORTANT INFO
- The Photorhabdus luminescens luxCDABE operon in the registry. It's not well characterised
Plasmid experiment
- Vibrio plasmid experiment -possible source of lux operon
- [1] - Transformation protocol for students from basics - includes several plasmids
- Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?
Transformation Experiment to test Firefly luciferase in registry
- Firefly luciferase is in the registry as BBa_I712019, and have been sent to us as part of the spring 2010 distribution.
- We need to find a new vector with a bacterial ribosyme binding site, initiator, and a stop codon. Having searched via the catalog in the plasmids section, then in expression plasmids, we have selected a constitutive plasmid:
- The plasmid selected is BBa_J13002, which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009.
Wavelengths
548 nm http://pubs.acs.org/doi/pdf/10.1021/bi7015052
613 nm http://www.promega.com/pnotes/85/10904_11/10904_11.pdf
