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Team:Cambridge/References/ProjectBioluminescence/Luciferase
From 2010.igem.org
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(→Photinus Pyralis (Firefly Luciferase)) |
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==Mutant (EPIC) luciferase (P. pyralis)== | ==Mutant (EPIC) luciferase (P. pyralis)== | ||
*[http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence] | *[http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence] | ||
| + | *[http://www.ncbi.nlm.nih.gov/pubmed/10529195 Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color] | ||
| + | *[http://www.ncbi.nlm.nih.gov/pubmed/12044905 Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis] | ||
==Bacterial Bioluminescence== | ==Bacterial Bioluminescence== | ||
Revision as of 09:56, 23 July 2010
Mutant (EPIC) luciferase (P. pyralis)
- Mutant (EPIC) Luciferase (P.pyralis) Sequence
- Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color
- Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
Bacterial Bioluminescence
- Vibrio Fischeri
- Vibrio Harveyi
- Genetics of luxCDABE (adapted from wikipedia)
- 2006 attempts at BioBricking the lux operon
- Notes
- The lux wiki from parts registry
- NCBI Lux operon sequence
- parts registry lux operon -currently waiting on an email to see how useful/available this part is
- IMPORTANT INFO
Plasmid experiment
- Vibrio plasmid experiment -possible source of lux operon
- [1] - Transformation protocol for students from basics - includes several plasmids
- Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?
