Team:Cambridge/References/ProjectBioluminescence/Luciferase

From 2010.igem.org

(Difference between revisions)
(Bacterial Bioluminescence)
(Transformation Experiment to test Firefly luciferase in registry)
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*We need to find a new vector with a bacterial ribosyme binding site, initiator, and a stop codon. Having searched via the catalog in the plasmids section, then in expression plasmids, we have selected a constitutive plasmid:
*We need to find a new vector with a bacterial ribosyme binding site, initiator, and a stop codon. Having searched via the catalog in the plasmids section, then in expression plasmids, we have selected a constitutive plasmid:
** The plasmid selected is [http://www.partsregistry.org/Part:BBa_J13002 BBa_J13002], which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009.
** The plasmid selected is [http://www.partsregistry.org/Part:BBa_J13002 BBa_J13002], which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009.
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==Wavelengths==
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548 nm http://departments.kings.edu/biology/lux/bacterial.html
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Revision as of 12:03, 28 July 2010