Bioluminescence

The Plan

• Characterise the transfer function of PoPs + CHBT --> Light

The Theory

• The Luciferin Cycle:

Increasing Light Emission

• Paper on isolation of H-LRE and G-LRE

Previous attempts to improve light production of luciferase reaction Pyruvate orthophosphate dikinase (PPDK) converts AMP + PPi to ATP (AMP + PPi + Phosphoenol Pyruvate -> ATP + Pyruvate + Phosphate) => light emission intensified in presence of low ATP concentration

• Enhanced luciferase activity through cytodine nucleotides
• Gene chimerisation to improve practical usefulness of firefly luciferase
• Genetically modified firefly luciferase - the EPIC luciferase

Sequences

• A-LRE (P.pyralis) Sequence
• Mutant Luciferase (P.pyralis) Sequence