Team:Cambridge/References/ProjectBioluminescence

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(The Plan)
 
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=Bioluminescence=
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==The Plan==
==The Plan==
* Characterise the transfer function of PoPs + CHBT  --> Light
* Characterise the transfer function of PoPs + CHBT  --> Light
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* Our Characterisation should look like [http://www.nature.com/nature/journal/v434/n7037/abs/nature03461.html this].
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* Our Characterisation should look like [http://www.nature.com/nbt/journal/v26/n7/abs/nbt1413.html this].
==The Theory==
==The Theory==
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==ADLA==
==ADLA==
The Abrupt Decline in Luciferase Activity is a phenomenon described for bacterial luciferase in E.coli in stationary phase of growth (see [http://www.springerlink.com/content/w73k840k27866462/fulltext.pdf Koga et al. 2005]) The paper identifies two mutants, HupA and an N-terminal deletion of H-NS for which ADLA does not occur. hns205, the mutation necessary for quiescence is a C-terminal deletion. We should find out whether the phenotypes are the same, which would be sweet if we ended up doing quiescence as well.
The Abrupt Decline in Luciferase Activity is a phenomenon described for bacterial luciferase in E.coli in stationary phase of growth (see [http://www.springerlink.com/content/w73k840k27866462/fulltext.pdf Koga et al. 2005]) The paper identifies two mutants, HupA and an N-terminal deletion of H-NS for which ADLA does not occur. hns205, the mutation necessary for quiescence is a C-terminal deletion. We should find out whether the phenotypes are the same, which would be sweet if we ended up doing quiescence as well.
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Latest revision as of 12:16, 7 October 2010