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Team:Cambridge/References/ProjectBioluminescence
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[http://www3.interscience.wiley.com/cgi-bin/fulltext/120766166/PDFSTART Chan et al. 1991] | [http://www3.interscience.wiley.com/cgi-bin/fulltext/120766166/PDFSTART Chan et al. 1991] | ||
| + | ==ADLA== | ||
| + | The Abrupt Decline in Luciferase Activity is a phenomenon described for bacterial luciferase in E.coli in stationary phase of growth (see [http://www.springerlink.com/content/w73k840k27866462/fulltext.pdf Koga et al. 2005]) The paper identifies two mutants, HupA and an N-terminal deletion of H-NS for which ADLA does not occur. hns205, the mutation necessary for quiescence is a C-terminal deletion. We should find out whether the phenotypes are the same, which would be sweet if we ended up doing quiescence as well. | ||
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Revision as of 15:36, 23 July 2010
Bioluminescence
The Plan
- Characterise the transfer function of PoPs + CHBT --> Light
The Theory
- The Luciferin Cycle:
Amazing mutant
paper on cloning the lux operon from Ponyfish Photobacterium leiognathi into E.coli, by the E.coli mutant 43R the luminescence output could be increased dramatically to near native levels. --> find out what 43R does! Chan et al. 1991
ADLA
The Abrupt Decline in Luciferase Activity is a phenomenon described for bacterial luciferase in E.coli in stationary phase of growth (see Koga et al. 2005) The paper identifies two mutants, HupA and an N-terminal deletion of H-NS for which ADLA does not occur. hns205, the mutation necessary for quiescence is a C-terminal deletion. We should find out whether the phenotypes are the same, which would be sweet if we ended up doing quiescence as well.
