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Team:Cambridge/References/ProjectBioluminescence
From 2010.igem.org
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Pyruvate orthophosphate dikinase (PPDK) converts AMP + PPi to ATP (AMP + PPi + Phosphoenol Pyruvate -> ATP + Pyruvate + Phosphate) => light emission intensified in presence of low ATP concentration | Pyruvate orthophosphate dikinase (PPDK) converts AMP + PPi to ATP (AMP + PPi + Phosphoenol Pyruvate -> ATP + Pyruvate + Phosphate) => light emission intensified in presence of low ATP concentration | ||
*[http://www.ncbi.nlm.nih.gov/pubmed/1332531 Enhanced luciferase activity through cytodine nucleotides] | *[http://www.ncbi.nlm.nih.gov/pubmed/1332531 Enhanced luciferase activity through cytodine nucleotides] | ||
| + | *[http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase] | ||
*[http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase] | *[http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase] | ||
Revision as of 13:58, 22 July 2010
Bioluminescence
The Plan
- Characterise the transfer function of PoPs + CHBT --> Light
- The Luciferin Cycle:
Increasing Light Emission
Previous attempts to improve light production of luciferase reaction Pyruvate orthophosphate dikinase (PPDK) converts AMP + PPi to ATP (AMP + PPi + Phosphoenol Pyruvate -> ATP + Pyruvate + Phosphate) => light emission intensified in presence of low ATP concentration
- Enhanced luciferase activity through cytodine nucleotides
- Gene chimerisation to improve practical usefulness of firefly luciferase
- Genetically modified firefly luciferase - the EPIC luciferase
Sequences
