Team:Cambridge/Quiescence Notes

From 2010.igem.org

(Difference between revisions)
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*[http://apps.isiknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=12&SID=W11BA3C7bKeHC5PbfoE&page=1&doc=1&colname=WOS ColE1 multimer formation triggers inhibition of Escherichia coli cell division.]
*[http://apps.isiknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=12&SID=W11BA3C7bKeHC5PbfoE&page=1&doc=1&colname=WOS ColE1 multimer formation triggers inhibition of Escherichia coli cell division.]
*[http://books.google.com/books?hl=en&lr=&id=OKn60-HZisUC&oi=fnd&pg=PA19&dq=hn-s+nucleoid+bacillus&ots=rstjtFpQ2Z&sig=15uulXPnHz-yyXMvMKRrTXcLk3U#v=onepage&q=h-ns&f=false book about bac genomes]
*[http://books.google.com/books?hl=en&lr=&id=OKn60-HZisUC&oi=fnd&pg=PA19&dq=hn-s+nucleoid+bacillus&ots=rstjtFpQ2Z&sig=15uulXPnHz-yyXMvMKRrTXcLk3U#v=onepage&q=h-ns&f=false book about bac genomes]
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Literally no documentation of h-ns and rcd in bacillus.
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If we do quiescence, will have to be in e coli with bacillus as a side project. Have to get over IP issues with ucam + e coli. Implementation in ecoli will be fairly easy as it is a working system - all we need to do is brick it, and get it working.

Revision as of 10:07, 15 July 2010

  • [http://mic.sgmjournals.org/cgi/reprint/155/8/2676.pdf The role of FIS in the Rcd checkpoint and stable maintenance of plasmid ColE1] ie good as the toggle control thingy
  • [http://www3.interscience.wiley.com/cgi-bin/fulltext/119127730/PDFSTART Timing, self-control and a sense of direction are the secrets of multicopy plasmid stability]
  • [http://aem.asm.org/cgi/reprint/65/6/2710.pdf The quiescent-cell expression system for protein synthesis in Escherichia coli]
  • [http://apps.isiknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=12&SID=W11BA3C7bKeHC5PbfoE&page=1&doc=1&colname=WOS ColE1 multimer formation triggers inhibition of Escherichia coli cell division.]
  • [http://books.google.com/books?hl=en&lr=&id=OKn60-HZisUC&oi=fnd&pg=PA19&dq=hn-s+nucleoid+bacillus&ots=rstjtFpQ2Z&sig=15uulXPnHz-yyXMvMKRrTXcLk3U#v=onepage&q=h-ns&f=false book about bac genomes]

Literally no documentation of h-ns and rcd in bacillus.

If we do quiescence, will have to be in e coli with bacillus as a side project. Have to get over IP issues with ucam + e coli. Implementation in ecoli will be fairly easy as it is a working system - all we need to do is brick it, and get it working.