Team:Cambridge/Protocols/ColonyPCR

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(Difference between revisions)
(New page: {{:Team:Cambridge/Templates/header}} {{:Team:Cambridge/Protocol Menu}} =Colony PCR= ==Materials== *2x Phusion Mastermic *Primers *Nuclease free H2O *Cell culture! ==Method== *Pick a sing...)
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**X = 1µL
**X = 1µL
**Y = 65°C
**Y = 65°C
 +
*[http://www.finnzymes.com/pdf/f531_f532_phusion_highfidelity_pcr_mastermix_datasheet_1_9_low.pdf Datasheet for the 2X Phusion Mastermix]
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Revision as of 15:06, 23 August 2010