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Team:Cambridge/PhosphoreumOligos
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ribA Reverse Primer: | ribA Reverse Primer: | ||
| - | + | :Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC | |
| - | + | :Structure: one hairpin in suffix/coding region | |
| - | + | :Tm: 56.27 deg | |
| - | + | :dG: 0.48 kcal/mol | |
luxE Reverse Primer: | luxE Reverse Primer: | ||
Revision as of 16:32, 13 August 2010
Phosphoreum Oligos
ribA Reverse Primer:
- Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
- Structure: one hairpin in suffix/coding region
- Tm: 56.27 deg
- dG: 0.48 kcal/mol
luxE Reverse Primer:
Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
Structure: one hairpin in suffix
Tm: 56.59 deg
dG: 0.57 kcal/mol
Pbad Reverse C-end: Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC Structure: one small hairpin in gene Tm: 53.68 deg dG: 2.30 kcal/mol
Lux C forward primer: Sequence: AGGAGATTGTATGATAAAGAAAAT Tm: 56.26 dG: 1.98
lux A(XbaI)
forward TACTGGACActagaGCATCATT Tm=58.46 dG=1.53
reverse AATGATGCtctagTGTCCAGTA Tm=58.46 dG=2.53
lux E(SpeI)
forward GTAGTGGTacCagtGGTGTAAA Tm=58.54 dG=1.41
reverse TTTACACCactGgtACCACTAC Tm=58.54 dG=1.25
lux G(XbaI) forward TGCCTGTCctagaAAACTTT Tm=58.56 dG=1.77
reverse AAAGTTTtctagGACAGGCA Tm=58.56 dG=1.33
ribB (EcoRI i)
forward GAATATGGTgaGttcAATATGATC Tm=58.39 dG=1.91
reverse GATCATATTgaaCtcACCATATTC Tm=58.39 dG=1.91
ribB (EcoRI ii)
forward TGAGCCgaaCtcTCAAAC Tm=59.64 dG=1.26
reverse GTTTGAgaGttcGGCTCA Tm=59.64 dG=1.16
