Team:Cambridge/PhosphoreumOligos

From 2010.igem.org

(Difference between revisions)

Latest revision as of 16:43, 13 August 2010

Phosphoreum Oligos

ribA Reverse Primer:

Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC

Structure: one hairpin in suffix/coding region

Tm: 56.27 deg

dG: 0.48 kcal/mol

luxE Reverse Primer:

Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA

Structure: one hairpin in suffix

Tm: 56.59 deg

dG: 0.57 kcal/mol

Pbad Reverse C-end:

Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC

Structure: one small hairpin in gene

Tm: 53.68 deg

dG: 2.30 kcal/mol

Lux C forward primer:

Sequence: AGGAGATTGTATGATAAAGAAAAT

Tm: 56.26

dG: 1.98

lux A(XbaI) forward:

Sequence: TACTGGACActagaGCATCATT

Tm: 58.46

dG: 1.53

lux A(XbaI) reverse:

Sequence: AATGATGCtctagTGTCCAGTA

Tm: 58.46

dG: 2.53

lux E(SpeI) Forward:

Sequence: GTAGTGGTacCagtGGTGTAAA

Tm: 58.54

dG: 1.41

lux E(SpeI) reverse:

Sequence: TTTACACCactGgtACCACTAC

Tm: 58.54

dG: 1.25

lux G(XbaI) forward:

Sequence: TGCCTGTCctagaAAACTTT

Tm: 58.56

dG: 1.77

luxG(XbaI) reverse:

Sequence: AAAGTTTtctagGACAGGCA

Tm: 58.56

dG: 1.33

ribB (EcoRI i) forward:

Sequence: GAATATGGTgaGttcAATATGATC

Tm: 58.39

dG: 1.91

ribB (EcoRI i) reverse:

Sequence: GATCATATTgaaCtcACCATATTC

Tm: 58.39

dG: 1.91

ribB (EcoRI ii) forward:

Sequence: TGAGCCgaaCtcTCAAAC

Tm: 59.64

dG: 1.26

ribB (EcoRI ii) reverse:

Sequence: GTTTGAgaGttcGGCTCA

Tm: 59.64

dG: 1.16

@@ Line 2: / Line 2: @@
 ribA Reverse Primer:
-	Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
-	Structure: one hairpin in suffix/coding region
+:Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
-	Tm: 56.27 deg
-	dG: 0.48 kcal/mol
+:Structure: one hairpin in suffix/coding region
+:Tm: 56.27 deg
+:dG: 0.48 kcal/mol
 luxE Reverse Primer:
-	Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
-	Structure: one hairpin in suffix
+:Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
-	Tm: 56.59 deg
-	dG: 0.57 kcal/mol
+:Structure: one hairpin in suffix
+:Tm: 56.59 deg
+:dG: 0.57 kcal/mol
 Pbad Reverse C-end:
-	Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
-	Structure: one small hairpin in gene
+:Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
-	Tm: 53.68 deg
-	dG: 2.30 kcal/mol
+:Structure: one small hairpin in gene
+:Tm: 53.68 deg
+:dG: 2.30 kcal/mol
 Lux C forward primer:
-Sequence: AGGAGATTGTATGATAAAGAAAAT
-Tm: 56.26
-dG: 1.98
+:Sequence: AGGAGATTGTATGATAAAGAAAAT
+:Tm: 56.26
+:dG: 1.98
+lux A(XbaI) forward:
+:Sequence: TACTGGACActagaGCATCATT
+:Tm: 58.46
+:dG: 1.53
+lux A(XbaI) reverse:
+:Sequence: AATGATGCtctagTGTCCAGTA
+:Tm: 58.46
+:dG: 2.53
+lux E(SpeI) Forward:
+:Sequence: GTAGTGGTacCagtGGTGTAAA
+:Tm: 58.54
+:dG: 1.41
+lux E(SpeI) reverse:
+:Sequence: TTTACACCactGgtACCACTAC
+:Tm: 58.54
+:dG: 1.25
+lux G(XbaI) forward:
+:Sequence: TGCCTGTCctagaAAACTTT
+:Tm: 58.56
+:dG: 1.77
+luxG(XbaI) reverse:
+:Sequence: AAAGTTTtctagGACAGGCA
+:Tm: 58.56
-lux A(XbaI)
+:dG: 1.33
-forward
-TACTGGACActagaGCATCATT
-Tm=58.46
-dG=1.53
-reverse
+ribB (EcoRI i) forward:
-AATGATGCtctagTGTCCAGTA
-Tm=58.46
-dG=2.53
+:Sequence: GAATATGGTgaGttcAATATGATC
+:Tm: 58.39
-lux E(SpeI)
+:dG: 1.91
-forward
+ribB (EcoRI i) reverse:
-GTAGTGGTacCagtGGTGTAAA
-Tm=58.54
-dG=1.41
-reverse
+:Sequence: GATCATATTgaaCtcACCATATTC
-TTTACACCactGgtACCACTAC
-Tm=58.54
-dG=1.25
+:Tm: 58.39
+:dG: 1.91
-lux G(XbaI)
-forward
-TGCCTGTCctagaAAACTTT
-Tm=58.56
-dG=1.77
-reverse
+ribB (EcoRI ii) forward:
-AAAGTTTtctagGACAGGCA
-Tm=58.56
-dG=1.33
+:Sequence: TGAGCCgaaCtcTCAAAC
-ribB (EcoRI i)
+:Tm: 59.64
-forward
+:dG: 1.26
-GAATATGGTgaGttcAATATGATC
-Tm=58.39
-dG=1.91
-reverse
+ribB (EcoRI ii) reverse:
-GATCATATTgaaCtcACCATATTC
-Tm=58.39
-dG=1.91
-ribB (EcoRI ii)
+:Sequence: GTTTGAgaGttcGGCTCA
-forward
+:Tm: 59.64
-TGAGCCgaaCtcTCAAAC
-Tm=59.64
-dG=1.26
-reverse
+:dG: 1.16
-GTTTGAgaGttcGGCTCA
-Tm=59.64
-dG=1.16