Team:Cambridge/Oligos

From 2010.igem.org

(Difference between revisions)
(Notes on alterations to the SLOCK pHK724 clone)
(Notes on alterations to the SLOCK pHK724 clone)
Line 10: Line 10:
===Notes on alterations to the SLOCK pHK724 clone===
===Notes on alterations to the SLOCK pHK724 clone===
-
*The sequence which SLOCK sent us is just the sequence which is found on [http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1 NCBI]
+
*The sequence which SLOCK sent us is just the sequence which is found on [http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1 NCBI] (found by sequence alignment of the two sequences)
*We do not know exactly what is in the plasmid, maybe some information can be found in the references [[Team:Cambridge/References/ProjectBioluminescence/Luciferase#Plasmid_experiment | here]]
*We do not know exactly what is in the plasmid, maybe some information can be found in the references [[Team:Cambridge/References/ProjectBioluminescence/Luciferase#Plasmid_experiment | here]]
 +
*From the annotated start of the lux ICDABEG operon from NCBI there are no restriction sites in conflict with the BioBrick standards.
 +
*Editing ideas:
 +
**Remove I and G regions from the operon (are these useful?)
 +
**Attach standard plasmid
===luxICDABE operon===
===luxICDABE operon===

Revision as of 12:12, 9 August 2010