Team:Cambridge/Oligos

From 2010.igem.org

(Difference between revisions)
(Firefly Oligos to be ordered)
(Notes on alterations to the SLOCK pHK724 clone)
 
(8 intermediate revisions not shown)
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[http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20I0500 Pbad Promoter Part]
[http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20I0500 Pbad Promoter Part]
 +
[http://www.fasebj.org/cgi/reprint/7/11/1016 Phosphoreum LuxL gene upstream of LuxC]
[http://www.fasebj.org/cgi/reprint/7/11/1016 Phosphoreum LuxL gene upstream of LuxC]
Line 31: Line 32:
[[Team:Cambridge/OligoOrderVibrio13.08.10 | Oligo Order for Vibrio Fischeri on 13.08.10]]
[[Team:Cambridge/OligoOrderVibrio13.08.10 | Oligo Order for Vibrio Fischeri on 13.08.10]]
 +
 +
[[Team:Cambridge/OligoOrderVibrio17.08.10 | Oligo Order for Vibrio Fischeri on 17.08.12]]
===Firefly Oligos to be ordered===
===Firefly Oligos to be ordered===
-
[[Team:Cambridge/mutagenesisprimers17.08.10 | Mutagenesis Oligos to be ordered before DNA 2.0 comes through]]
+
*[[Team:Cambridge/mutagenesisprimers17.08.10 | Mutagenesis Oligos to be ordered before DNA 2.0 comes through]]
-
[[Team:Cambridge/biobrickingoligosfirefly | Oligos to biobrick our DNA 2.0 order]]
+
*[[Team:Cambridge/biobrickingoligosfirefly | Oligos to biobrick our DNA 2.0 order]]
-
=lux oligos=
+
===Our first attempts at oligo design (a little excessive)===
-
==luxI ==      
+
-
===forward===
+
-
gaattcgcggccgcttctagagAAGGGAGGTTGGTATGACTAT
+
-
dG=0.11
+
-
Tm=58.45
+
-
===reverse coding===
+
[[Team:Cambridge/Biobrickingluxoperon | Oligos to BioBrick the whole lux operon]] (never ordered as too expensive)
-
atgatcatcgccggcgacgtcTTAATTTAAGACTGCTTTTTTAAACT
+
-
dG=-0.34
+
-
Tm=57.3
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcATAATATACTTAGTATTTAAAATAAATTAATGATTTA
+
-
dG=-0.34
+
-
Tm=56.83
+
 +
[[Team:Cambridge/luxICDABEoperon | luxICDABE operon sequence]] from NCBI
 +
[[Team:Cambridge/LuxR | LuxR sequence]] from NCBI
-
==luxC==
+
==Thioesterase==
-
===forward===      
+
-
gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
+
-
Tm=56.07
+
-
dG=0.76
+
-
===reverse coding===
+
ygcB gene from E. Coli K12 to over-express thioesterase:
-
atgatcatcgccggcgacgtcCTATGGGACAAAAACTAAAAAC
+
GTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATGAAGATACCGATGCCGGTGGTGTGGTGTACCACGCCAGTTACGTCGCTTTTTATGAAA
-
dG=-0.77
+
GAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTA
-
Tm=56.58
+
CGCACCTGCGCGGCTCGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAG
-
===reverse RBS===
+
AATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGC
-
atgatcatcgccggcgacgtcTAACTATGGGACAAAAACTAAA
+
AGTGA
-
dG=-0.34
+
-
Tm=55.31
+
 +
Forward Primer:
 +
gaattcgcggccgcttctagaGTGAATACAACGCTGTTTC
 +
Complement (for Gibson):
-
==luxD==
+
GAAACAGCGTTGTATTCACtctagaagcggccgcgaattc
-
===forward===
+
-
gaattcgcggccgcttctagagAAGGAAATTATATGAAAGATGAAA
+
-
Tm=57.08
+
-
dG=0.11
+
-
===reverse coding===
+
Bacward primer:
-
atgatcatcgccggcgacgtcTTAAGCCAATTCTAATAATTCATT
+
-
dG=-0.34
+
-
Tm=57.19
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcTTGGTGATTCTGTTTATTTAAG
+
-
dG= -0.34
+
-
Tm=55.69
+
 +
ctgcagcggccgctactagtaTCACTGCTTAAACTCCG
 +
Complement:
-
 
+
CGGAGTTTAAGCAGTGAtactagtagcggccgctgcag
-
==luxA==
+
-
===forward===
+
-
gaattcgcggccgcttctagagAAAGGAATAGAGTATGAAGTTTGG
+
-
Tm=58.8
+
-
dG=0.76
+
-
 
+
-
===reverse coding===
+
-
atgatcatcgccggcgacgtcTTATTTAGGTTCTTTTAAGAAAGGAG
+
-
dG=-0.34
+
-
Tm=58.66
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcTTATCTCTAGTATCAAATAAGTAATTTATTTAGG
+
-
dG=-0.34
+
-
Tm=58.84
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
==luxB==
+
-
===forward===
+
-
gaattcgcggccgcttctagagTAAGGAACAAGTTATGAAATTTG
+
-
Tm=57.09
+
-
dG=0.47
+
-
 
+
-
===reverse coding===
+
-
atgatcatcgccggcgacgtcTTATGGTAAATTCATTTCGATT
+
-
dG=-0.34
+
-
Tm=57.2
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcAATACCTAATATCAACAGAAATTTAAA
+
-
dG=-0.34
+
-
Tm=56.89
+
-
 
+
-
 
+
-
 
+
-
 
+
-
==luxE==
+
-
===forward===
+
-
gaattcgcggccgcttctagagACTGGAGAGGGTATGACTGT
+
-
Tm=58.15
+
-
dG=0.76
+
-
 
+
-
===reverse coding===
+
-
atgatcatcgccggcgacgtcTTAATCCTTGATATTCTTTTGTATG
+
-
dG=-0.34
+
-
Tm=57.5
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
+
-
dG=-0.34
+
-
Tm=57.96
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
==luxG==
+
-
===forward===
+
-
gaattcgcggccgcttctagagAAGGATTAAGTTATGATTGTTGA
+
-
Tm=56.52
+
-
dG=0.11
+
-
 
+
-
===reverse coding===
+
-
atgatcatcgccggcgacgtcTTATACGTATGCAAAAGCATC
+
-
dG=-1.21
+
-
Tm=57.48
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcAAAGCCGGATTAATAATCTG
+
-
dG=-3.57
+
-
Tm=57.82
+
-
 
+
-
alternative ===reverse RBS===
+
-
atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
+
-
dG=-0.34
+
-
Tm=57.36
+
-
 
+
-
===luxICDABE operon===
+
-
gtcgaccttcctggttcagagcctcatatccatattacgaccacttcaat
+
-
gggcgatcaaaaagtacattacatgattttttgcccaacagaaaaagcct
+
-
ctcatttagagcagcttattcgtcaagatttcatggaaatgtatgagatc
+
-
aattttccacaaaaaacagagtaacaaagagaaaaacctataggtataaa
+
-
gctttacttacgtacttaatttttaaagtatgggcaatcaattgctcctg
+
-
ttaaaattgctttagaaatactttggcagcggtttgttgtattgagtttc
+
-
atttgcgcattggttaaatggaaagtgacagtacgctcactgcagcctaa
+
-
tatttttgaaatatcccaagagctttttccttcgcatgcccacgctaaac
+
-
attctttttctcttttggttaaatcgttgtttgatttattatttgctata
+
-
tttatttttcgataattatcaactagagaaggaacaattaatggtatgtt
+
-
catacacgcatgtaaaaataaactatctatatagttgtctttttctgaat
+
-
gtgcaaaactaagcattccgaagccattgttagccgtatgaatagggaaa
+
-
ctaaacccagtgataagacctgatgttttcgcttctttaattacatttgg
+
-
agattttttatttacagcattgttttcaaatatattccaattaattggtg
+
-
aatgattggagttagaataatctactataggatcatattttattaaatta
+
-
gcgtcatcataatattgcctccattttttagggtaattatctagaattga
+
-
aatatcagatttaaccatagaatgaggataaatgatcgcgagtaaataat
+
-
attcacaatgtaccattttagtcatatcagataagcattgattaatatca
+
-
ttattgcttctacaagctttaattttattaattattctgtatgtgtcgtc
+
-
ggcatttatgtttttcatacccatctctttatccttacctattgtttgtc
+
-
gcaagttttgcgtgttatatatcattaaaacggtaatggattgacatttg
+
-
attctaataaattggatttttgtcacactattgtatcgctgggaatacaa
+
-
ttacttaacataagcacctgtaggatcgtacaggtttacgcaagaaaatg
+
-
gtttgttatagtcgaataaacgcaagggaggttggtatgactataatgat
+
-
aaaaaaatcggattttttggcaattccatcggaggagtataaaggtattc
+
-
taagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagtt
+
-
gtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaata
+
-
tatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttat
+
-
tacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgctt
+
-
ggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
+
-
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaatta
+
-
caatgaaactatttgaagctatatataaacacgctgttagtcaaggtatt
+
-
acagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcg
+
-
tattaaagttccttgtcatcgtattggagacaaagaaattcatgtattag
+
-
gtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa
+
-
aaagcagtcttaaattaatgttgttaaatcattaatttattttaaatact
+
-
aagtatattatagggaaaataatgaataaatgtattccaatgataattaa
+
-
tggaatgattcaagattttgataattatgcatataaagaagttaaactaa
+
-
ataatgataatagagtaaaattatctgtcattactgaaagttcagtttca
+
-
aaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgt
+
-
gaattttttatataccgttggtcaacgatggaaaagtgaagaatataatc
+
-
ggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgat
+
-
gaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaa
+
-
aagtgctttgtatgacattgttaattatgatttgggctctatacacgtat
+
-
tagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaa
+
-
ggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgac
+
-
atctattttgcgtgctattttaacaaaaaatgagtgcattattaaaactt
+
-
cgtcttcagatccttttactgcaaacgctttagtttccagttttattgat
+
-
gttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggcc
+
-
gcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacg
+
-
tggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatat
+
-
tcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaag
+
-
tattattgaagctcctaaagatatagaagccgcagcaatgggggttgctc
+
-
atgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtt
+
-
tattatataggagataatttacctttatttttaaatgaacttgaaaaaca
+
-
gctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatg
+
-
aaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatat
+
-
gaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctac
+
-
aaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaag
+
-
tatctgatattaaagagataattccttttgttaataaaaatagaacacaa
+
-
actgtttctatttatccttgggaagcgtcattaaaatatcgagataaatt
+
-
agcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattt
+
-
tcagagttggaggggctcatgattcattatctcctctccagtacctagtt
+
-
aggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgc
+
-
ggttgaaatcgaacaaacacgttacttagaggaagataagtttttagttt
+
-
ttgtcccatagttaaaggaaattatatgaaagatgaaagtgcttttttta
+
-
cgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgg
+
-
gaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttat
+
-
tgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagt
+
-
atttatctactaacggttttcatgtcattcgctacgattctttgcatcat
+
-
gttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaa
+
-
aaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaac
+
-
gaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggta
+
-
gtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaa
+
-
tcttagagatacattagaaaaagcattggagtatgactatttgcaattac
+
-
ctatttcagagttaccagaagatcttgactttgaaggtcataatttagga
+
-
tcggaggtctttgttacagattgctttaaacataattgggacacgttaga
+
-
ctcgacacttaatagtgttaaaggattagcgattccatttattgctttta
+
-
ctgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcatt
+
-
gatagcattgaatctagtaattgtaagctctattcgctaattggaagttc
+
-
acatgatcttggggaaaatttggttgtattaagaaatttttatcaatcag
+
-
tgacgaaggcagccttagcattagatgatggtttattggatttagagata
+
-
gacattattgaacctcgatttgaggacgttacaagtattactgttaagga
+
-
gcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaaa
+
-
taaacagaatcaccaaaaaggaatagagtatgaagtttggaaatatttgt
+
-
ttttcgtatcaaccaccaggtgaaactcataagcaagtaatggatcgctt
+
-
tgttcggcttggtatcgcctcagaagaggtagggtttgatacatattgga
+
-
ccttagaacatcattttacagagtttggtcttacgggaaatttatttgtt
+
-
gctgcggctaacctgttaggaagaactaaaacattaaatgttggcactat
+
-
gggggttgttattccgacagcacacccagttcgacagttagaagacgttt
+
-
tattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcga
+
-
gggctataccataaagattttcgagtatttggtgttgatatggaagagtc
+
-
tcgagcaattactcaaaatttctaccagatgataatggaaagcttacaga
+
-
caggaaccattagctctgatagtgattacattcaatttcctaaggttgat
+
-
gtatatcccaaagtgtactcaaaaaatgtaccaacctgtatgactgctga
+
-
gtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttc
+
-
ttagttggattattggtactaatgaaaaaaaagcacagatggaactctat
+
-
aatgaaattgcgacagaatatggtcatgatatatctaaaatagatcattg
+
-
tatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatg
+
-
tttgtcgggagtttctgaaaaattggtatgactcatatgtaaatgcgacc
+
-
aatatctttaatgatagcaatcaaactcgtggttatgattatcataaagg
+
-
tcaatggcgtgattttgttttacaaggacatacaaacaccaatcgacgtg
+
-
ttgattatagcaatggtattaaccctgtaggcactcctgagcagtgtatt
+
-
gaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatg
+
-
cggatttgaagctaatggaactgaagatgaaataattgcttccatgcgac
+
-
gctttatgacacaagtcgctcctttcttaaaagaacctaaataaattact
+
-
tatttgatactagagataataaggaacaagttatgaaatttggattattt
+
-
tttctaaactttcagaaagatggaataacatctgaagaaacgttggataa
+
-
tatggtaaagactgtcacgttaattgattcaactaaatatcattttaata
+
-
ctgcctttgttaatgaacatcacttttcaaaaaatggtattgttggagca
+
-
cctattaccgcagctggttttttattagggttaacaaataaattacatat
+
-
tggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcag
+
-
aagaagccagtttattagatcaaatgtcagagggacgcttcattcttggt
+
-
tttagtgactgcgaaagtgatttcgaaatggaattttttagacgtcatat
+
-
ctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacg
+
-
cattaactacaggttattgtcatccccaaaacgacttttatgattttcca
+
-
aaggtttcaattaatccacactgttacagtgagaatggacctaagcaata
+
-
tgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcac
+
-
tgcctttaacatttaagtgggaggataatttagaaaccaaagaacgctat
+
-
gcaattctatataataaaacagcacaacaatatggtattgatatttcgga
+
-
tgttgatcatcaattaactgtaattgcgaacttaaatgctgatagaagta
+
-
cggctcaagaagaagtgagagaatacttaaaagactatatcactgaaact
+
-
taccctcaaatggacagagatgaaaaaattaactgcattattgaagagaa
+
-
tgcagttgggtctcatgatgactattatgaatcgacaaaattagcagtgg
+
-
aaaaaacagggtctaaaaatattttattatcctttgaatcaatgtccgat
+
-
attaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaat
+
-
gaatttaccataataaaattaaaggcaatttctatattagattgcctttt
+
-
taaatttctgttgatattaggtattactggagagggtatgactgtccata
+
-
ctgaatataaaagaaatcaaatcattgctagttcagaaattgatgatctt
+
-
atctttatgacgaaaccacaagagtggtcatttgaagagcaaaaagaaat
+
-
acgggataaattagttcgtgaggctttttattttcactacaatagaaatg
+
-
aagaatatagaaattattgtatcaatcagcatgtgagtgataatttacac
+
-
actattgatgaaatacccgtgtttccaacatctgtttttaaatataagaa
+
-
attacatactgtcacagccgaggacattgaaaattggtatacaagtagtg
+
-
gaactcgtggagtaaaaagtcatattgcacgtgatcgtcttagcattgaa
+
-
cgcttgcttggttctgtcaacttcggaatgaaatacgttggagattggtt
+
-
tgagcatcaaatggaattgataaatttaggaccagatagattcaatacaa
+
-
ataatatttggtttaaatatgtcatgagtttggtcgagttactttatccg
+
-
actgaatttacagttgataatgacaaaatagattttgaaaaaacagtaaa
+
-
acatctatttagaattaagaatagtaaaaaagacatttgcttaattgggc
+
-
caccattttttgtgtatcttttgtgccaatatatgaaagaaaacaatatt
+
-
gaatttaaaggaggagatagagtacatattattactggtggaggatggaa
+
-
atctaatcagaatgactctttagatcgtgctgattttaatcaattattaa
+
-
tggatactttccaactcgacaagattaatcaaattagagatacctttaat
+
-
caagttgagcttaatacttgtttttttgaagatgaatttcaaagaaaaca
+
-
tgttccaccgtgggtatatgctcgggctcttgatcctgaaaccttgaaac
+
-
ccgtagcagatggtgagatcgggttgttaagttatatggacgcctcatca
+
-
actgcttaccctgcttttattgttactgatgatatcggtattgtaaaaga
+
-
aattagagaaccagatccttacccaggggtaactgttgagattgttcggc
+
-
gcttaaatacacgtgcgcaaaaaggatgcgcgctctctatggctaatgtc
+
-
atacaaaagaatatcaaggattaagttatgattgttgatggtagagtttc
+
-
aaaaatagtattagcgtcaataagaaataatatatataaggtatttatta
+
-
ctgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacg
+
-
attaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaa
+
-
ttacgaaatagaattgcatattggtagttcgaatagagactgctcattgg
+
-
atattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgag
+
-
ttagatgctccccatggaaacgcttggttacggtctgaaagtaataaccc
+
-
attgctattaattgcgggaggtacaggtttatcatatataaatagcattc
+
-
taacaaattgcttaaataggaatatacctcaagatatttatctttactgg
+
-
ggagtaaaagacagttctcttttgtatgaagatgaagagttactaaactt
+
-
atcactaaacaacaaaaactttcattatattcctgttattgaagataaaa
+
-
gtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaa
+
-
gattttactgatcttacttattttgatatttatgtttgtggacccttcat
+
-
gatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagt
+
-
cagaacagatgtttgccgatgcttttgcatacgtataaagagaatataaa
+
-
aagccagattattaatccggcttttttattatttcaatgtgtccaagggt
+
-
aaccaagttacttcaactcccgcctgagagaacatatcttggctcacttg
+
-
aattttttcaccccaacgagataagaagtcttcagtttgttcagggcaat
+
-
atacttttgatattcctgtttggatgatttttgctgcgcagtttggacaa
+
-
gggaagtgagtaacccaaatatcgcaaccttctaaatcacgcttggcaaa
+
-
taaaatggcattttcttcagcatgaagtgtttttaaatatttaatttcac
+
-
gttcatctgtatctgcactgtctgatacaccatgtgggtagccattaaat
+
-
ccaacagaaacaatacggttatgcttggtgattactgcaccaacttgagt
+
-
cgac
+
-
 
+
-
===luxR===
+
-
ATGAAAAACATAAATGCCGACGACACATACAGAATAATTAATAAAATTAA
+
-
AGCTTGTAGAAGCAATAATGATATAATCAATGCTTATCTGATATGACTAA
+
-
AATGGTACATTGTGAATATTATTTATTCGCGATCATTTATCCTCATTCTA
+
-
TGGTTAAATCTGATATTTCAATCTAGATAATTACCCTAAAAAATGGAGGC
+
-
AATATTATGATGACGCTAATTTAATAAAATATGATCCTATAGTAGATTAT
+
-
TCTAACTCCAATCATTCACCAATTAATTGGAATATATTTGAAAACAATGC
+
-
TGTAAATAAAAAATCTCCAAATGTAATTAAGAAGCGAAAACATCAGGTCT
+
-
TATCACTGGGTTTAGTTTCCCTATTCATACGGCTAACAATGCCTTCGGAA
+
-
TGCTTAGTTTTGCACATTCAGAAAAAGACAACTATATAGATAGTTTATTT
+
-
TTACATGCGTGTATGAACATACCATTAATTGTTCCTTCTCTAGTGTATAA
+
-
TTATCGAAAAATAAATATAGCAAATAATAAATCAAACAACGATTTAACCA
+
-
AAAGAGAAAAAGAATGTTTAGCGTGGGCATGCGAAGGAAAAGGCTCTTGG
+
-
GATATTTCAAAAATATTAGGCTGCAGTGAGCGTACTGTCACTTTCCATTT
+
-
AACCAATGCGCAAATGAAACTCAATACACCAAACCGCTGCCAAAGTATTT
+
-
CTAAAGCAATTTTAACAGGAGCAATTGATTGCCCATACTTTAAAAATTAA
+
-
 
+
-
==Thioesterase==
+
-
 
+
-
ygcB gene from E. Coli K12 to over-express thioesterase:
+
-
GTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATGAAGATACCGATGCCGGTGGTGTGGTGTACCACGCCAGTTACGTCGCTTTTTATGAAA
+
-
GAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTA
+
-
CGCACCTGCGCGGCTCGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAG
+
-
AATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGC
+
-
AGTGA
+

Latest revision as of 10:27, 18 August 2010