Team:Cambridge/Oligos

From 2010.igem.org

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(Our first attempts at oligo design (a little excessive))
(Notes on alterations to the SLOCK pHK724 clone)
 
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[http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20I0500 Pbad Promoter Part]
[http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20I0500 Pbad Promoter Part]
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[http://www.fasebj.org/cgi/reprint/7/11/1016 Phosphoreum LuxL gene upstream of LuxC]
[http://www.fasebj.org/cgi/reprint/7/11/1016 Phosphoreum LuxL gene upstream of LuxC]
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[[Team:Cambridge/OligoOrderVibrio13.08.10 | Oligo Order for Vibrio Fischeri on 13.08.10]]
[[Team:Cambridge/OligoOrderVibrio13.08.10 | Oligo Order for Vibrio Fischeri on 13.08.10]]
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[[Team:Cambridge/OligoOrderVibrio17.08.10 | Oligo Order for Vibrio Fischeri on 17.08.12]]
===Firefly Oligos to be ordered===
===Firefly Oligos to be ordered===
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[[Team:Cambridge/mutagenesisprimers17.08.10 | Mutagenesis Oligos to be ordered before DNA 2.0 comes through]]
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*[[Team:Cambridge/mutagenesisprimers17.08.10 | Mutagenesis Oligos to be ordered before DNA 2.0 comes through]]
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[[Team:Cambridge/biobrickingoligosfirefly | Oligos to biobrick our DNA 2.0 order]]
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*[[Team:Cambridge/biobrickingoligosfirefly | Oligos to biobrick our DNA 2.0 order]]
===Our first attempts at oligo design (a little excessive)===
===Our first attempts at oligo design (a little excessive)===
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AATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGC
AATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGC
AGTGA
AGTGA
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Forward Primer:
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gaattcgcggccgcttctagaGTGAATACAACGCTGTTTC
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Complement (for Gibson):
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GAAACAGCGTTGTATTCACtctagaagcggccgcgaattc
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Bacward primer:
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ctgcagcggccgctactagtaTCACTGCTTAAACTCCG
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Complement:
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CGGAGTTTAAGCAGTGAtactagtagcggccgctgcag

Latest revision as of 10:27, 18 August 2010