Team:Cambridge/Oligos

From 2010.igem.org

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(Notes on alterations to the SLOCK pHK724 clone)
 
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[http://www.ncbi.nlm.nih.gov/nuccore/166797857 DQ988873]
[http://www.ncbi.nlm.nih.gov/nuccore/166797857 DQ988873]
 +
[http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20I0500 Pbad Promoter Part]
 +
 +
[http://www.fasebj.org/cgi/reprint/7/11/1016 Phosphoreum LuxL gene upstream of LuxC]
 +
 +
[[Team:Cambridge/PhosphoreumOligos | The phosphoreum oligos we ordered]]
===Notes on alterations to the SLOCK pHK724 clone===
===Notes on alterations to the SLOCK pHK724 clone===
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*How to design a [http://partsregistry.org/Ribosome_Binding_Sites/Design RBS]
*How to design a [http://partsregistry.org/Ribosome_Binding_Sites/Design RBS]
-
=lux oligos=
+
[[Team:Cambridge/OligoOrderVibrio13.08.10 | Oligo Order for Vibrio Fischeri on 13.08.10]]
-
==luxI ==      
+
-
===forward===
+
-
gaattcgcggccgcttctagagAAGGGAGGTTGGTATGACTAT
+
-
dG=0.11
+
-
Tm=58.45
+
-
===reverse coding===
+
[[Team:Cambridge/OligoOrderVibrio17.08.10 | Oligo Order for Vibrio Fischeri on 17.08.12]]
-
atgatcatcgccggcgacgtcTTAATTTAAGACTGCTTTTTTAAACT
+
-
dG=-0.34
+
-
Tm=57.3
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcATAATATACTTAGTATTTAAAATAAATTAATGATTTA
+
-
dG=-0.34
+
-
Tm=56.83
+
 +
===Firefly Oligos to be ordered===
 +
*[[Team:Cambridge/mutagenesisprimers17.08.10 | Mutagenesis Oligos to be ordered before DNA 2.0 comes through]]
 +
*[[Team:Cambridge/biobrickingoligosfirefly | Oligos to biobrick our DNA 2.0 order]]
 +
===Our first attempts at oligo design (a little excessive)===
-
==luxC==
+
[[Team:Cambridge/Biobrickingluxoperon | Oligos to BioBrick the whole lux operon]] (never ordered as too expensive)
-
===forward===      
+
-
gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
+
-
Tm=56.07
+
-
dG=0.76
+
-
===reverse coding===
+
[[Team:Cambridge/luxICDABEoperon | luxICDABE operon sequence]] from NCBI
-
atgatcatcgccggcgacgtcCTATGGGACAAAAACTAAAAAC
+
-
dG=-0.77
+
-
Tm=56.58
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcTAACTATGGGACAAAAACTAAA
+
-
dG=-0.34
+
-
Tm=55.31
+
 +
[[Team:Cambridge/LuxR | LuxR sequence]] from NCBI
 +
==Thioesterase==
 +
ygcB gene from E. Coli K12 to over-express thioesterase:
 +
GTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATGAAGATACCGATGCCGGTGGTGTGGTGTACCACGCCAGTTACGTCGCTTTTTATGAAA
 +
GAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTA
 +
CGCACCTGCGCGGCTCGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAG
 +
AATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGC
 +
AGTGA
-
==luxD==
+
Forward Primer:
-
===forward===
+
-
gaattcgcggccgcttctagagAAGGAAATTATATGAAAGATGAAA
+
-
Tm=57.08
+
-
dG=0.11
+
-
===reverse coding===
+
gaattcgcggccgcttctagaGTGAATACAACGCTGTTTC
-
atgatcatcgccggcgacgtcTTAAGCCAATTCTAATAATTCATT
+
-
dG=-0.34
+
-
Tm=57.19
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcTTGGTGATTCTGTTTATTTAAG
+
-
dG= -0.34
+
-
Tm=55.69
+
 +
Complement (for Gibson):
 +
GAAACAGCGTTGTATTCACtctagaagcggccgcgaattc
 +
Bacward primer:
-
==luxA==
+
ctgcagcggccgctactagtaTCACTGCTTAAACTCCG
-
===forward===
+
-
gaattcgcggccgcttctagagAAAGGAATAGAGTATGAAGTTTGG
+
-
Tm=58.8
+
-
dG=0.76
+
-
===reverse coding===
+
Complement:
-
atgatcatcgccggcgacgtcTTATTTAGGTTCTTTTAAGAAAGGAG
+
-
dG=-0.34
+
-
Tm=58.66
+
-
===reverse RBS===
+
-
atgatcatcgccggcgacgtcTTATCTCTAGTATCAAATAAGTAATTTATTTAGG
+
-
dG=-0.34
+
-
Tm=58.84
+
 +
CGGAGTTTAAGCAGTGAtactagtagcggccgctgcag
-
 
-
 
-
==luxB==
 
-
===forward===
 
-
gaattcgcggccgcttctagagTAAGGAACAAGTTATGAAATTTG
 
-
Tm=57.09
 
-
dG=0.47
 
-
 
-
===reverse coding===
 
-
atgatcatcgccggcgacgtcTTATGGTAAATTCATTTCGATT
 
-
dG=-0.34
 
-
Tm=57.2
 
-
===reverse RBS===
 
-
atgatcatcgccggcgacgtcAATACCTAATATCAACAGAAATTTAAA
 
-
dG=-0.34
 
-
Tm=56.89
 
-
 
-
 
-
 
-
 
-
==luxE==
 
-
===forward===
 
-
gaattcgcggccgcttctagagACTGGAGAGGGTATGACTGT
 
-
Tm=58.15
 
-
dG=0.76
 
-
 
-
===reverse coding===
 
-
atgatcatcgccggcgacgtcTTAATCCTTGATATTCTTTTGTATG
 
-
dG=-0.34
 
-
Tm=57.5
 
-
===reverse RBS===
 
-
atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
 
-
dG=-0.34
 
-
Tm=57.96
 
-
 
-
 
-
 
-
 
-
 
-
==luxG==
 
-
===forward===
 
-
gaattcgcggccgcttctagagAAGGATTAAGTTATGATTGTTGA
 
-
Tm=56.52
 
-
dG=0.11
 
-
 
-
===reverse coding===
 
-
atgatcatcgccggcgacgtcTTATACGTATGCAAAAGCATC
 
-
dG=-1.21
 
-
Tm=57.48
 
-
===reverse RBS===
 
-
atgatcatcgccggcgacgtcAAAGCCGGATTAATAATCTG
 
-
dG=-3.57
 
-
Tm=57.82
 
-
 
-
alternative ===reverse RBS===
 
-
atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
 
-
dG=-0.34
 
-
Tm=57.36
 
-
 
-
===luxICDABE operon===
 
-
gtcgaccttcctggttcagagcctcatatccatattacgaccacttcaat
 
-
gggcgatcaaaaagtacattacatgattttttgcccaacagaaaaagcct
 
-
ctcatttagagcagcttattcgtcaagatttcatggaaatgtatgagatc
 
-
aattttccacaaaaaacagagtaacaaagagaaaaacctataggtataaa
 
-
gctttacttacgtacttaatttttaaagtatgggcaatcaattgctcctg
 
-
ttaaaattgctttagaaatactttggcagcggtttgttgtattgagtttc
 
-
atttgcgcattggttaaatggaaagtgacagtacgctcactgcagcctaa
 
-
tatttttgaaatatcccaagagctttttccttcgcatgcccacgctaaac
 
-
attctttttctcttttggttaaatcgttgtttgatttattatttgctata
 
-
tttatttttcgataattatcaactagagaaggaacaattaatggtatgtt
 
-
catacacgcatgtaaaaataaactatctatatagttgtctttttctgaat
 
-
gtgcaaaactaagcattccgaagccattgttagccgtatgaatagggaaa
 
-
ctaaacccagtgataagacctgatgttttcgcttctttaattacatttgg
 
-
agattttttatttacagcattgttttcaaatatattccaattaattggtg
 
-
aatgattggagttagaataatctactataggatcatattttattaaatta
 
-
gcgtcatcataatattgcctccattttttagggtaattatctagaattga
 
-
aatatcagatttaaccatagaatgaggataaatgatcgcgagtaaataat
 
-
attcacaatgtaccattttagtcatatcagataagcattgattaatatca
 
-
ttattgcttctacaagctttaattttattaattattctgtatgtgtcgtc
 
-
ggcatttatgtttttcatacccatctctttatccttacctattgtttgtc
 
-
gcaagttttgcgtgttatatatcattaaaacggtaatggattgacatttg
 
-
attctaataaattggatttttgtcacactattgtatcgctgggaatacaa
 
-
ttacttaacataagcacctgtaggatcgtacaggtttacgcaagaaaatg
 
-
gtttgttatagtcgaataaacgcaagggaggttggtatgactataatgat
 
-
aaaaaaatcggattttttggcaattccatcggaggagtataaaggtattc
 
-
taagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagtt
 
-
gtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaata
 
-
tatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttat
 
-
tacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgctt
 
-
ggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
 
-
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaatta
 
-
caatgaaactatttgaagctatatataaacacgctgttagtcaaggtatt
 
-
acagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcg
 
-
tattaaagttccttgtcatcgtattggagacaaagaaattcatgtattag
 
-
gtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa
 
-
aaagcagtcttaaattaatgttgttaaatcattaatttattttaaatact
 
-
aagtatattatagggaaaataatgaataaatgtattccaatgataattaa
 
-
tggaatgattcaagattttgataattatgcatataaagaagttaaactaa
 
-
ataatgataatagagtaaaattatctgtcattactgaaagttcagtttca
 
-
aaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgt
 
-
gaattttttatataccgttggtcaacgatggaaaagtgaagaatataatc
 
-
ggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgat
 
-
gaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaa
 
-
aagtgctttgtatgacattgttaattatgatttgggctctatacacgtat
 
-
tagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaa
 
-
ggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgac
 
-
atctattttgcgtgctattttaacaaaaaatgagtgcattattaaaactt
 
-
cgtcttcagatccttttactgcaaacgctttagtttccagttttattgat
 
-
gttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggcc
 
-
gcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacg
 
-
tggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatat
 
-
tcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaag
 
-
tattattgaagctcctaaagatatagaagccgcagcaatgggggttgctc
 
-
atgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtt
 
-
tattatataggagataatttacctttatttttaaatgaacttgaaaaaca
 
-
gctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatg
 
-
aaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatat
 
-
gaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctac
 
-
aaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaag
 
-
tatctgatattaaagagataattccttttgttaataaaaatagaacacaa
 
-
actgtttctatttatccttgggaagcgtcattaaaatatcgagataaatt
 
-
agcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattt
 
-
tcagagttggaggggctcatgattcattatctcctctccagtacctagtt
 
-
aggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgc
 
-
ggttgaaatcgaacaaacacgttacttagaggaagataagtttttagttt
 
-
ttgtcccatagttaaaggaaattatatgaaagatgaaagtgcttttttta
 
-
cgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgg
 
-
gaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttat
 
-
tgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagt
 
-
atttatctactaacggttttcatgtcattcgctacgattctttgcatcat
 
-
gttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaa
 
-
aaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaac
 
-
gaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggta
 
-
gtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaa
 
-
tcttagagatacattagaaaaagcattggagtatgactatttgcaattac
 
-
ctatttcagagttaccagaagatcttgactttgaaggtcataatttagga
 
-
tcggaggtctttgttacagattgctttaaacataattgggacacgttaga
 
-
ctcgacacttaatagtgttaaaggattagcgattccatttattgctttta
 
-
ctgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcatt
 
-
gatagcattgaatctagtaattgtaagctctattcgctaattggaagttc
 
-
acatgatcttggggaaaatttggttgtattaagaaatttttatcaatcag
 
-
tgacgaaggcagccttagcattagatgatggtttattggatttagagata
 
-
gacattattgaacctcgatttgaggacgttacaagtattactgttaagga
 
-
gcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaaa
 
-
taaacagaatcaccaaaaaggaatagagtatgaagtttggaaatatttgt
 
-
ttttcgtatcaaccaccaggtgaaactcataagcaagtaatggatcgctt
 
-
tgttcggcttggtatcgcctcagaagaggtagggtttgatacatattgga
 
-
ccttagaacatcattttacagagtttggtcttacgggaaatttatttgtt
 
-
gctgcggctaacctgttaggaagaactaaaacattaaatgttggcactat
 
-
gggggttgttattccgacagcacacccagttcgacagttagaagacgttt
 
-
tattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcga
 
-
gggctataccataaagattttcgagtatttggtgttgatatggaagagtc
 
-
tcgagcaattactcaaaatttctaccagatgataatggaaagcttacaga
 
-
caggaaccattagctctgatagtgattacattcaatttcctaaggttgat
 
-
gtatatcccaaagtgtactcaaaaaatgtaccaacctgtatgactgctga
 
-
gtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttc
 
-
ttagttggattattggtactaatgaaaaaaaagcacagatggaactctat
 
-
aatgaaattgcgacagaatatggtcatgatatatctaaaatagatcattg
 
-
tatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatg
 
-
tttgtcgggagtttctgaaaaattggtatgactcatatgtaaatgcgacc
 
-
aatatctttaatgatagcaatcaaactcgtggttatgattatcataaagg
 
-
tcaatggcgtgattttgttttacaaggacatacaaacaccaatcgacgtg
 
-
ttgattatagcaatggtattaaccctgtaggcactcctgagcagtgtatt
 
-
gaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatg
 
-
cggatttgaagctaatggaactgaagatgaaataattgcttccatgcgac
 
-
gctttatgacacaagtcgctcctttcttaaaagaacctaaataaattact
 
-
tatttgatactagagataataaggaacaagttatgaaatttggattattt
 
-
tttctaaactttcagaaagatggaataacatctgaagaaacgttggataa
 
-
tatggtaaagactgtcacgttaattgattcaactaaatatcattttaata
 
-
ctgcctttgttaatgaacatcacttttcaaaaaatggtattgttggagca
 
-
cctattaccgcagctggttttttattagggttaacaaataaattacatat
 
-
tggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcag
 
-
aagaagccagtttattagatcaaatgtcagagggacgcttcattcttggt
 
-
tttagtgactgcgaaagtgatttcgaaatggaattttttagacgtcatat
 
-
ctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacg
 
-
cattaactacaggttattgtcatccccaaaacgacttttatgattttcca
 
-
aaggtttcaattaatccacactgttacagtgagaatggacctaagcaata
 
-
tgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcac
 
-
tgcctttaacatttaagtgggaggataatttagaaaccaaagaacgctat
 
-
gcaattctatataataaaacagcacaacaatatggtattgatatttcgga
 
-
tgttgatcatcaattaactgtaattgcgaacttaaatgctgatagaagta
 
-
cggctcaagaagaagtgagagaatacttaaaagactatatcactgaaact
 
-
taccctcaaatggacagagatgaaaaaattaactgcattattgaagagaa
 
-
tgcagttgggtctcatgatgactattatgaatcgacaaaattagcagtgg
 
-
aaaaaacagggtctaaaaatattttattatcctttgaatcaatgtccgat
 
-
attaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaat
 
-
gaatttaccataataaaattaaaggcaatttctatattagattgcctttt
 
-
taaatttctgttgatattaggtattactggagagggtatgactgtccata
 
-
ctgaatataaaagaaatcaaatcattgctagttcagaaattgatgatctt
 
-
atctttatgacgaaaccacaagagtggtcatttgaagagcaaaaagaaat
 
-
acgggataaattagttcgtgaggctttttattttcactacaatagaaatg
 
-
aagaatatagaaattattgtatcaatcagcatgtgagtgataatttacac
 
-
actattgatgaaatacccgtgtttccaacatctgtttttaaatataagaa
 
-
attacatactgtcacagccgaggacattgaaaattggtatacaagtagtg
 
-
gaactcgtggagtaaaaagtcatattgcacgtgatcgtcttagcattgaa
 
-
cgcttgcttggttctgtcaacttcggaatgaaatacgttggagattggtt
 
-
tgagcatcaaatggaattgataaatttaggaccagatagattcaatacaa
 
-
ataatatttggtttaaatatgtcatgagtttggtcgagttactttatccg
 
-
actgaatttacagttgataatgacaaaatagattttgaaaaaacagtaaa
 
-
acatctatttagaattaagaatagtaaaaaagacatttgcttaattgggc
 
-
caccattttttgtgtatcttttgtgccaatatatgaaagaaaacaatatt
 
-
gaatttaaaggaggagatagagtacatattattactggtggaggatggaa
 
-
atctaatcagaatgactctttagatcgtgctgattttaatcaattattaa
 
-
tggatactttccaactcgacaagattaatcaaattagagatacctttaat
 
-
caagttgagcttaatacttgtttttttgaagatgaatttcaaagaaaaca
 
-
tgttccaccgtgggtatatgctcgggctcttgatcctgaaaccttgaaac
 
-
ccgtagcagatggtgagatcgggttgttaagttatatggacgcctcatca
 
-
actgcttaccctgcttttattgttactgatgatatcggtattgtaaaaga
 
-
aattagagaaccagatccttacccaggggtaactgttgagattgttcggc
 
-
gcttaaatacacgtgcgcaaaaaggatgcgcgctctctatggctaatgtc
 
-
atacaaaagaatatcaaggattaagttatgattgttgatggtagagtttc
 
-
aaaaatagtattagcgtcaataagaaataatatatataaggtatttatta
 
-
ctgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacg
 
-
attaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaa
 
-
ttacgaaatagaattgcatattggtagttcgaatagagactgctcattgg
 
-
atattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgag
 
-
ttagatgctccccatggaaacgcttggttacggtctgaaagtaataaccc
 
-
attgctattaattgcgggaggtacaggtttatcatatataaatagcattc
 
-
taacaaattgcttaaataggaatatacctcaagatatttatctttactgg
 
-
ggagtaaaagacagttctcttttgtatgaagatgaagagttactaaactt
 
-
atcactaaacaacaaaaactttcattatattcctgttattgaagataaaa
 
-
gtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaa
 
-
gattttactgatcttacttattttgatatttatgtttgtggacccttcat
 
-
gatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagt
 
-
cagaacagatgtttgccgatgcttttgcatacgtataaagagaatataaa
 
-
aagccagattattaatccggcttttttattatttcaatgtgtccaagggt
 
-
aaccaagttacttcaactcccgcctgagagaacatatcttggctcacttg
 
-
aattttttcaccccaacgagataagaagtcttcagtttgttcagggcaat
 
-
atacttttgatattcctgtttggatgatttttgctgcgcagtttggacaa
 
-
gggaagtgagtaacccaaatatcgcaaccttctaaatcacgcttggcaaa
 
-
taaaatggcattttcttcagcatgaagtgtttttaaatatttaatttcac
 
-
gttcatctgtatctgcactgtctgatacaccatgtgggtagccattaaat
 
-
ccaacagaaacaatacggttatgcttggtgattactgcaccaacttgagt
 
-
cgac
 
-
 
-
===luxR===
 
-
ATGAAAAACATAAATGCCGACGACACATACAGAATAATTAATAAAATTAA
 
-
AGCTTGTAGAAGCAATAATGATATAATCAATGCTTATCTGATATGACTAA
 
-
AATGGTACATTGTGAATATTATTTATTCGCGATCATTTATCCTCATTCTA
 
-
TGGTTAAATCTGATATTTCAATCTAGATAATTACCCTAAAAAATGGAGGC
 
-
AATATTATGATGACGCTAATTTAATAAAATATGATCCTATAGTAGATTAT
 
-
TCTAACTCCAATCATTCACCAATTAATTGGAATATATTTGAAAACAATGC
 
-
TGTAAATAAAAAATCTCCAAATGTAATTAAGAAGCGAAAACATCAGGTCT
 
-
TATCACTGGGTTTAGTTTCCCTATTCATACGGCTAACAATGCCTTCGGAA
 
-
TGCTTAGTTTTGCACATTCAGAAAAAGACAACTATATAGATAGTTTATTT
 
-
TTACATGCGTGTATGAACATACCATTAATTGTTCCTTCTCTAGTGTATAA
 
-
TTATCGAAAAATAAATATAGCAAATAATAAATCAAACAACGATTTAACCA
 
-
AAAGAGAAAAAGAATGTTTAGCGTGGGCATGCGAAGGAAAAGGCTCTTGG
 
-
GATATTTCAAAAATATTAGGCTGCAGTGAGCGTACTGTCACTTTCCATTT
 
-
AACCAATGCGCAAATGAAACTCAATACACCAAACCGCTGCCAAAGTATTT
 
-
CTAAAGCAATTTTAACAGGAGCAATTGATTGCCCATACTTTAAAAATTAA
 
-
 
-
==Thioesterase==
 
-
 
-
ygcB gene from E. Coli K12 to over-express thioesterase:
 
-
GTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATGAAGATACCGATGCCGGTGGTGTGGTGTACCACGCCAGTTACGTCGCTTTTTATGAAA
 
-
GAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTA
 
-
CGCACCTGCGCGGCTCGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAG
 
-
AATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGC
 
-
AGTGA
 
-
 
-
Forward primer:
 
-
gaattcgcggccgcttctagagGTGAATACAACGCTGTTTCG prefix + 20bp
 
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Latest revision as of 10:27, 18 August 2010