Team:Cambridge/OligoOrderVibrio13.08.10
From 2010.igem.org
BioBrick Prefix:
If following part is coding or contains ATG:
gaattcgcggccgcttctag
Otherwise:
gaattcgcggccgcttctagag
BioBrick Suffix:
tactagtagcggccgctgcag
Pbad Forward Prefix Primer:
Sequence: gaattcgcggccgcttctagagTTATGACAACTTGACGG
Structure: one hairpin in gene
Tm: 52.95 deg
dG: 1.00 kcal/mol
Pbad Reverse Oend
Sequence: tagtcataccaacctcccttgcgGCTAGCCCAAAAAAAC
Structure: one hairpin in gene
Tm: 53.68 deg
dG: 1.11 kcal/mol
luxR forward primer
Sequence: ATGAAAAACATAAATGCCG
Tm: 58.06 deg
dG: 1.59 kcal/mol
luxR reverse Iend Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC Tm: 58.54 deg dG: 0.34 kcal/mol
luxI forward primer Sequence: AAGGGAGGTTGGTATGACTATA Tm: 58.52 deg dG: 2.41 kcal/mol
luxG reverse suffix primer (finishing before the final terminator) Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA Tm: 57.36 deg dG: -0.34 kcal/mol
luxO forward primer Sequence:AGGGAAAATAATGAATAAATGTATT Tm: 57.29 deg dG: 2.13 kcal/mol
luxO reverse suffix primer (finishing at RBS of luxG) Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT Tm: 57.96 deg dG: -0.34 kcal/mol
Pbad Reverse Rend
Sequence: ttcatctagtatttctcctctttGCTAGCCCAAAAAAAC
Structure: one hairpin in gene
Tm: 53.68 deg
dG: 1.83 kcal/mol
luxO forward prefix primer Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT Tm: 56.07 deg dG: 0.76 kcal/mol