Team:Cambridge/OligoOrderVibrio13.08.10

From 2010.igem.org

(Difference between revisions)
 
Line 45: Line 45:
 +
luxR forward primer
luxR forward primer
Line 52: Line 53:
dG: 1.59 kcal/mol
dG: 1.59 kcal/mol
 +
 +
luxR reverse Iend
luxR reverse Iend
Line 60: Line 63:
dG: 0.34 kcal/mol
dG: 0.34 kcal/mol
 +
Line 69: Line 73:
dG: 2.41 kcal/mol
dG: 2.41 kcal/mol
 +
 +
luxG reverse suffix primer (finishing before the final terminator)
luxG reverse suffix primer (finishing before the final terminator)
Line 77: Line 83:
dG: -0.34 kcal/mol  
dG: -0.34 kcal/mol  
 +
 +
luxO forward primer
luxO forward primer
Line 85: Line 93:
dG: 2.13 kcal/mol
dG: 2.13 kcal/mol
 +
 +
luxO reverse suffix primer (finishing at RBS of luxG)
luxO reverse suffix primer (finishing at RBS of luxG)
Line 93: Line 103:
dG: -0.34 kcal/mol
dG: -0.34 kcal/mol
 +
 +
Pbad Reverse Rend
Pbad Reverse Rend
Line 103: Line 115:
dG: 1.83 kcal/mol
dG: 1.83 kcal/mol
 +
 +
luxO forward prefix primer
luxO forward prefix primer

Latest revision as of 11:47, 13 August 2010

BioBrick Prefix:

If following part is coding or contains ATG:

gaattcgcggccgcttctag

Otherwise:

gaattcgcggccgcttctagag


BioBrick Suffix:

tactagtagcggccgctgcag





Pbad Forward Prefix Primer:

Sequence: gaattcgcggccgcttctagagTTATGACAACTTGACGG

Structure: one hairpin in gene

Tm: 52.95 deg

dG: 1.00 kcal/mol


Pbad Reverse Oend

Sequence: tagtcataccaacctcccttgcgGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.11 kcal/mol


luxR forward primer

Sequence: ATGAAAAACATAAATGCCG

Tm: 58.06 deg

dG: 1.59 kcal/mol


luxR reverse Iend

Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC

Tm: 58.54 deg

dG: 0.34 kcal/mol


luxI forward primer

Sequence: AAGGGAGGTTGGTATGACTATA

Tm: 58.52 deg

dG: 2.41 kcal/mol


luxG reverse suffix primer (finishing before the final terminator)

Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA

Tm: 57.36 deg

dG: -0.34 kcal/mol


luxO forward primer

Sequence:AGGGAAAATAATGAATAAATGTATT

Tm: 57.29 deg

dG: 2.13 kcal/mol


luxO reverse suffix primer (finishing at RBS of luxG)

Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT

Tm: 57.96 deg

dG: -0.34 kcal/mol


Pbad Reverse Rend

Sequence: ttcatctagtatttctcctctttGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.83 kcal/mol


luxO forward prefix primer

Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT

Tm: 56.07 deg

dG: 0.76 kcal/mol