Team:Cambridge/OligoOrderVibrio13.08.10
From 2010.igem.org
EmilyKnott (Talk | contribs) |
EmilyKnott (Talk | contribs) |
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luxR forward primer | luxR forward primer | ||
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dG: 1.59 kcal/mol | dG: 1.59 kcal/mol | ||
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luxR reverse Iend | luxR reverse Iend | ||
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dG: 0.34 kcal/mol | dG: 0.34 kcal/mol | ||
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dG: 2.41 kcal/mol | dG: 2.41 kcal/mol | ||
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luxG reverse suffix primer (finishing before the final terminator) | luxG reverse suffix primer (finishing before the final terminator) | ||
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dG: -0.34 kcal/mol | dG: -0.34 kcal/mol | ||
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luxO forward primer | luxO forward primer | ||
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dG: 2.13 kcal/mol | dG: 2.13 kcal/mol | ||
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luxO reverse suffix primer (finishing at RBS of luxG) | luxO reverse suffix primer (finishing at RBS of luxG) | ||
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dG: -0.34 kcal/mol | dG: -0.34 kcal/mol | ||
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Pbad Reverse Rend | Pbad Reverse Rend | ||
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dG: 1.83 kcal/mol | dG: 1.83 kcal/mol | ||
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luxO forward prefix primer | luxO forward prefix primer |
Latest revision as of 11:47, 13 August 2010
BioBrick Prefix:
If following part is coding or contains ATG:
gaattcgcggccgcttctag
Otherwise:
gaattcgcggccgcttctagag
BioBrick Suffix:
tactagtagcggccgctgcag
Pbad Forward Prefix Primer:
Sequence: gaattcgcggccgcttctagagTTATGACAACTTGACGG
Structure: one hairpin in gene
Tm: 52.95 deg
dG: 1.00 kcal/mol
Pbad Reverse Oend
Sequence: tagtcataccaacctcccttgcgGCTAGCCCAAAAAAAC
Structure: one hairpin in gene
Tm: 53.68 deg
dG: 1.11 kcal/mol
luxR forward primer
Sequence: ATGAAAAACATAAATGCCG
Tm: 58.06 deg
dG: 1.59 kcal/mol
luxR reverse Iend
Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC
Tm: 58.54 deg
dG: 0.34 kcal/mol
luxI forward primer
Sequence: AAGGGAGGTTGGTATGACTATA
Tm: 58.52 deg
dG: 2.41 kcal/mol
luxG reverse suffix primer (finishing before the final terminator)
Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
Tm: 57.36 deg
dG: -0.34 kcal/mol
luxO forward primer
Sequence:AGGGAAAATAATGAATAAATGTATT
Tm: 57.29 deg
dG: 2.13 kcal/mol
luxO reverse suffix primer (finishing at RBS of luxG)
Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
Tm: 57.96 deg
dG: -0.34 kcal/mol
Pbad Reverse Rend
Sequence: ttcatctagtatttctcctctttGCTAGCCCAAAAAAAC
Structure: one hairpin in gene
Tm: 53.68 deg
dG: 1.83 kcal/mol
luxO forward prefix primer
Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
Tm: 56.07 deg
dG: 0.76 kcal/mol