Team:Cambridge/OligoOrderVibrio13.08.10

From 2010.igem.org

(Difference between revisions)
(New page: BioBrick Prefix: If following part is coding or contains ATG: gaattcgcggccgcttctag Otherwise: gaattcgcggccgcttctagag BioBrick Suffix: tactagtagcggccgctgcag Pb...)
 
(One intermediate revision not shown)
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 +
luxR forward primer
luxR forward primer
 +
Sequence: ATGAAAAACATAAATGCCG
Sequence: ATGAAAAACATAAATGCCG
 +
Tm: 58.06 deg  
Tm: 58.06 deg  
 +
dG: 1.59 kcal/mol
dG: 1.59 kcal/mol
 +
 +
luxR reverse Iend
luxR reverse Iend
 +
Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC
Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC
 +
Tm: 58.54 deg
Tm: 58.54 deg
 +
dG: 0.34 kcal/mol
dG: 0.34 kcal/mol
 +
 +
luxI forward primer
luxI forward primer
 +
Sequence: AAGGGAGGTTGGTATGACTATA
Sequence: AAGGGAGGTTGGTATGACTATA
 +
Tm: 58.52 deg  
Tm: 58.52 deg  
 +
dG: 2.41 kcal/mol
dG: 2.41 kcal/mol
 +
 +
luxG reverse suffix primer (finishing before the final terminator)
luxG reverse suffix primer (finishing before the final terminator)
 +
Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
 +
Tm: 57.36 deg  
Tm: 57.36 deg  
 +
dG: -0.34 kcal/mol  
dG: -0.34 kcal/mol  
 +
 +
luxO forward primer
luxO forward primer
 +
Sequence:AGGGAAAATAATGAATAAATGTATT
Sequence:AGGGAAAATAATGAATAAATGTATT
 +
Tm: 57.29 deg  
Tm: 57.29 deg  
 +
dG: 2.13 kcal/mol
dG: 2.13 kcal/mol
 +
 +
luxO reverse suffix primer (finishing at RBS of luxG)
luxO reverse suffix primer (finishing at RBS of luxG)
 +
Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
 +
Tm: 57.96 deg  
Tm: 57.96 deg  
 +
dG: -0.34 kcal/mol
dG: -0.34 kcal/mol
 +
 +
Pbad Reverse Rend
Pbad Reverse Rend
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dG: 1.83 kcal/mol
dG: 1.83 kcal/mol
 +
 +
luxO forward prefix primer
luxO forward prefix primer
 +
Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
 +
Tm: 56.07 deg  
Tm: 56.07 deg  
 +
dG: 0.76 kcal/mol
dG: 0.76 kcal/mol

Latest revision as of 11:47, 13 August 2010

BioBrick Prefix:

If following part is coding or contains ATG:

gaattcgcggccgcttctag

Otherwise:

gaattcgcggccgcttctagag


BioBrick Suffix:

tactagtagcggccgctgcag





Pbad Forward Prefix Primer:

Sequence: gaattcgcggccgcttctagagTTATGACAACTTGACGG

Structure: one hairpin in gene

Tm: 52.95 deg

dG: 1.00 kcal/mol


Pbad Reverse Oend

Sequence: tagtcataccaacctcccttgcgGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.11 kcal/mol


luxR forward primer

Sequence: ATGAAAAACATAAATGCCG

Tm: 58.06 deg

dG: 1.59 kcal/mol


luxR reverse Iend

Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC

Tm: 58.54 deg

dG: 0.34 kcal/mol


luxI forward primer

Sequence: AAGGGAGGTTGGTATGACTATA

Tm: 58.52 deg

dG: 2.41 kcal/mol


luxG reverse suffix primer (finishing before the final terminator)

Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA

Tm: 57.36 deg

dG: -0.34 kcal/mol


luxO forward primer

Sequence:AGGGAAAATAATGAATAAATGTATT

Tm: 57.29 deg

dG: 2.13 kcal/mol


luxO reverse suffix primer (finishing at RBS of luxG)

Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT

Tm: 57.96 deg

dG: -0.34 kcal/mol


Pbad Reverse Rend

Sequence: ttcatctagtatttctcctctttGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.83 kcal/mol


luxO forward prefix primer

Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT

Tm: 56.07 deg

dG: 0.76 kcal/mol