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Team:Cambridge/OligoOrderVibrio13.08.10
From 2010.igem.org
(New page: BioBrick Prefix: If following part is coding or contains ATG: gaattcgcggccgcttctag Otherwise: gaattcgcggccgcttctagag BioBrick Suffix: tactagtagcggccgctgcag Pb...) |
EmilyKnott (Talk | contribs) |
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luxR forward primer | luxR forward primer | ||
| + | |||
Sequence: ATGAAAAACATAAATGCCG | Sequence: ATGAAAAACATAAATGCCG | ||
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Tm: 58.06 deg | Tm: 58.06 deg | ||
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dG: 1.59 kcal/mol | dG: 1.59 kcal/mol | ||
luxR reverse Iend | luxR reverse Iend | ||
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Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC | Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC | ||
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Tm: 58.54 deg | Tm: 58.54 deg | ||
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dG: 0.34 kcal/mol | dG: 0.34 kcal/mol | ||
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luxI forward primer | luxI forward primer | ||
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Sequence: AAGGGAGGTTGGTATGACTATA | Sequence: AAGGGAGGTTGGTATGACTATA | ||
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Tm: 58.52 deg | Tm: 58.52 deg | ||
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dG: 2.41 kcal/mol | dG: 2.41 kcal/mol | ||
luxG reverse suffix primer (finishing before the final terminator) | luxG reverse suffix primer (finishing before the final terminator) | ||
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Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA | Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA | ||
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Tm: 57.36 deg | Tm: 57.36 deg | ||
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dG: -0.34 kcal/mol | dG: -0.34 kcal/mol | ||
luxO forward primer | luxO forward primer | ||
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Sequence:AGGGAAAATAATGAATAAATGTATT | Sequence:AGGGAAAATAATGAATAAATGTATT | ||
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Tm: 57.29 deg | Tm: 57.29 deg | ||
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dG: 2.13 kcal/mol | dG: 2.13 kcal/mol | ||
luxO reverse suffix primer (finishing at RBS of luxG) | luxO reverse suffix primer (finishing at RBS of luxG) | ||
| + | |||
Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT | Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT | ||
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Tm: 57.96 deg | Tm: 57.96 deg | ||
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dG: -0.34 kcal/mol | dG: -0.34 kcal/mol | ||
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luxO forward prefix primer | luxO forward prefix primer | ||
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Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT | Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT | ||
| + | |||
Tm: 56.07 deg | Tm: 56.07 deg | ||
| + | |||
dG: 0.76 kcal/mol | dG: 0.76 kcal/mol | ||
Revision as of 11:46, 13 August 2010
BioBrick Prefix:
If following part is coding or contains ATG:
gaattcgcggccgcttctag
Otherwise:
gaattcgcggccgcttctagag
BioBrick Suffix:
tactagtagcggccgctgcag
Pbad Forward Prefix Primer:
Sequence: gaattcgcggccgcttctagagTTATGACAACTTGACGG
Structure: one hairpin in gene
Tm: 52.95 deg
dG: 1.00 kcal/mol
Pbad Reverse Oend
Sequence: tagtcataccaacctcccttgcgGCTAGCCCAAAAAAAC
Structure: one hairpin in gene
Tm: 53.68 deg
dG: 1.11 kcal/mol
luxR forward primer
Sequence: ATGAAAAACATAAATGCCG
Tm: 58.06 deg
dG: 1.59 kcal/mol
luxR reverse Iend
Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC
Tm: 58.54 deg
dG: 0.34 kcal/mol
luxI forward primer
Sequence: AAGGGAGGTTGGTATGACTATA
Tm: 58.52 deg
dG: 2.41 kcal/mol
luxG reverse suffix primer (finishing before the final terminator)
Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
Tm: 57.36 deg
dG: -0.34 kcal/mol
luxO forward primer
Sequence:AGGGAAAATAATGAATAAATGTATT
Tm: 57.29 deg
dG: 2.13 kcal/mol
luxO reverse suffix primer (finishing at RBS of luxG)
Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
Tm: 57.96 deg
dG: -0.34 kcal/mol
Pbad Reverse Rend
Sequence: ttcatctagtatttctcctctttGCTAGCCCAAAAAAAC
Structure: one hairpin in gene
Tm: 53.68 deg
dG: 1.83 kcal/mol
luxO forward prefix primer
Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
Tm: 56.07 deg
dG: 0.76 kcal/mol
