http://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&feed=atom&action=historyTeam:Cambridge/Notebook/Week6 - Revision history2024-03-29T01:59:13ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=52942&oldid=prevTheos: Replacing page with '
{{:Team:Cambridge/Templates/HybridBook|num=6}}'2010-08-18T22:45:22Z<p>Replacing page with ' {{:Team:Cambridge/Templates/HybridBook|num=6}}'</p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Cambridge/Templates/headerMinimalprototype}}</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Cambridge/Templates/headerbar|colour=#fad72a|title=Notebook: Week 6}}</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">== Monday==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/<ins class="diffchange diffchange-inline">HybridBook</ins>|<ins class="diffchange diffchange-inline">num</ins>=<ins class="diffchange diffchange-inline">6</ins>}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/<del class="diffchange diffchange-inline">rightpic</del>|<del class="diffchange diffchange-inline">src</del>=<del class="diffchange diffchange-inline">Cambridge-16aug.jpg}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In the morning we checked the BMG microplate reader which had been run over the weekend and provided interesting growth curves, quantifying the extent to which adding chloramphenicol to the medium slowed growth (this was by c. 50%). Bill miniprepped lux R DNA from the registry. James told us that to use the standard Gibson protocol we needed 40bp of overlap, not the 20bp we planned which required rethinking oligos. Peter prepared a number of cultures to test the effect of lumazine on emissions.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In the evening Hannah, Peter, Anja and Theo combined take-aways and beer with primer design, which went surprisingly well.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">{{Team:Cambridge/Templates/Day|Day=Tuesday}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">{{:Team:Cambridge/Templates/rightpic|src=Cambridge-TuePic.jpg}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In the morning we sent off an order to Biolegio for oligo synthesis, in order to PCR out the Vibrio fischeri lux operon. Will also designed oligos for the site-directed mutagenesis of Luciola cruciata luciferase into different colours, ready from when it arrives from DNA 2.0.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We decided to have a synthetic lux operon synthesised, relieving HNS repression. Theo and Anja transformed TOP10 cells with Enhanced Yellow Fluorescent protein and a TetR repressed promoter from the registry, the latter will be used to control the firely operons. Will and Hannah set up an experiment in the BMG plate reader which was our first test of its ability to detect bioluminescent emissions.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">{{Team:Cambridge/Templates/Day|Day=Wednesday}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">{{:Team:Cambridge/Templates/rightpic|src=Camb-Notes.jpg}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Bill wrote a piece of software in Python to simplify primer design for Gibson Assembly. Theo and Hannah designed a synthetic V. fischeri lux operon with codon usage and GC content optimised. Will prepared DNA for use with the oligos we hope will arrive tomorrow. Paul analysed results from the BMG plate reader.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==Friday==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==Saturday==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==Sunday==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><html></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=52787&oldid=prevTheos at 18:07, 18 August 20102010-08-18T18:07:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Wednesday}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Wednesday}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Cambridge/Templates/rightpic|src=Camb-Notes.jpg}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Bill wrote a piece of software in Python to simplify primer design for Gibson Assembly. Theo and Hannah designed a synthetic V. fischeri lux operon with codon usage and GC content optimised. Will prepared DNA for use with the oligos we hope will arrive tomorrow. Paul analysed results from the BMG plate reader.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>=Thursday<del class="diffchange diffchange-inline">==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{Team:Cambridge/Templates/Day|Day</ins>=Thursday<ins class="diffchange diffchange-inline">}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Friday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Friday==</div></td></tr>
</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=52210&oldid=prevTheos at 22:46, 17 August 20102010-08-17T22:46:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Tuesday}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Tuesday}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Cambridge/Templates/rightpic|src=Cambridge-TuePic.jpg}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the morning we sent off an order to Biolegio for oligo synthesis, in order to PCR out the Vibrio fischeri lux operon. Will also designed oligos for the site-directed mutagenesis of Luciola cruciata luciferase into different colours, ready from when it arrives from DNA 2.0.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the morning we sent off an order to Biolegio for oligo synthesis, in order to PCR out the Vibrio fischeri lux operon. Will also designed oligos for the site-directed mutagenesis of Luciola cruciata luciferase into different colours, ready from when it arrives from DNA 2.0.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=52170&oldid=prevTheos at 20:32, 17 August 20102010-08-17T20:32:45Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Tuesday}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Tuesday}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">In the morning we sent off an order to Biolegio for oligo synthesis, in order to PCR out the Vibrio fischeri lux operon. Will also designed oligos for the site-directed mutagenesis of Luciola cruciata luciferase into different colours, ready from when it arrives from DNA 2.0.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We decided to have a synthetic lux operon synthesised, relieving HNS repression. Theo and Anja transformed TOP10 cells with Enhanced Yellow Fluorescent protein and a TetR repressed promoter from the registry, the latter will be used to control the firely operons. Will and Hannah set up an experiment in the BMG plate reader which was our first test of its ability to detect bioluminescent emissions.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Wednesday}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Wednesday}}</div></td></tr>
</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=51673&oldid=prevTheos: /* Monday */2010-08-16T23:30:14Z<p><span class="autocomment">Monday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the morning we checked the BMG microplate reader which had been run over the weekend and provided interesting growth curves, quantifying the extent to which adding chloramphenicol to the medium slowed growth (this was by c. 50%). Bill miniprepped lux R DNA from the registry. James told us that to use the standard Gibson protocol we needed 40bp of overlap, not the 20bp we planned which required rethinking oligos. Peter prepared a number of cultures to test the effect of lumazine on emissions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the morning we checked the BMG microplate reader which had been run over the weekend and provided interesting growth curves, quantifying the extent to which adding chloramphenicol to the medium slowed growth (this was by c. 50%). Bill miniprepped lux R DNA from the registry. James told us that to use the standard Gibson protocol we needed 40bp of overlap, not the 20bp we planned which required rethinking oligos. Peter prepared a number of cultures to test the effect of lumazine on emissions.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In the evening Hannah, Peter, Anja and Theo combined <del class="diffchange diffchange-inline">a </del>take-<del class="diffchange diffchange-inline">away </del>and beer with primer design, which went surprisingly well.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In the evening Hannah, Peter, Anja and Theo combined take-<ins class="diffchange diffchange-inline">aways </ins>and beer with primer design, which went surprisingly well.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=51657&oldid=prevTheos: /* Monday */2010-08-16T23:06:30Z<p><span class="autocomment">Monday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Monday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Monday==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:Cambridge/Templates/rightpic|src=Cambridge-16aug.jpg}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the morning we checked the BMG microplate reader which had been run over the weekend and provided interesting growth curves, quantifying the extent to which adding chloramphenicol to the medium slowed growth (this was by c. 50%). Bill miniprepped lux R DNA from the registry. James told us that to use the standard Gibson protocol we needed 40bp of overlap, not the 20bp we planned which required rethinking oligos. Peter prepared a number of cultures to test the effect of lumazine on emissions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the morning we checked the BMG microplate reader which had been run over the weekend and provided interesting growth curves, quantifying the extent to which adding chloramphenicol to the medium slowed growth (this was by c. 50%). Bill miniprepped lux R DNA from the registry. James told us that to use the standard Gibson protocol we needed 40bp of overlap, not the 20bp we planned which required rethinking oligos. Peter prepared a number of cultures to test the effect of lumazine on emissions.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the evening Hannah, Peter, Anja and Theo combined a take-away and beer with primer design, which went surprisingly well.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the evening Hannah, Peter, Anja and Theo combined a take-away and beer with primer design, which went surprisingly well.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">{{:Team:Cambridge/Templates/rightpic|src=Cambridge-16aug.jpg}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Tuesday}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Cambridge/Templates/Day|Day=Tuesday}}</div></td></tr>
</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=51656&oldid=prevTheos at 23:06, 16 August 20102010-08-16T23:06:19Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:06, 16 August 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>=Tuesday<del class="diffchange diffchange-inline">==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{Team:Cambridge/Templates/Day|Day</ins>=Tuesday<ins class="diffchange diffchange-inline">}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=<del class="diffchange diffchange-inline">=Wednesdy==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{Team:Cambridge/Templates/Day|Day</ins>=<ins class="diffchange diffchange-inline">Wednesday}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Thursday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Thursday==</div></td></tr>
</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=51655&oldid=prevTheos: /* Monday */2010-08-16T23:05:48Z<p><span class="autocomment">Monday</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:05, 16 August 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Monday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Monday==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">In the morning we checked the BMG microplate reader which had been run over the weekend and provided interesting growth curves, quantifying the extent to which adding chloramphenicol to the medium slowed growth (this was by c. 50%). Bill miniprepped lux R DNA from the registry. James told us that to use the standard Gibson protocol we needed 40bp of overlap, not the 20bp we planned which required rethinking oligos. Peter prepared a number of cultures to test the effect of lumazine on emissions.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">In the evening Hannah, Peter, Anja and Theo combined a take-away and beer with primer design, which went surprisingly well.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/rightpic|src=Cambridge-16aug.jpg}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/rightpic|src=Cambridge-16aug.jpg}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Tuesday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Tuesday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=51315&oldid=prevTheos at 11:28, 16 August 20102010-08-16T11:28:11Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 11:28, 16 August 2010</td>
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<td colspan="2" class="diff-lineno">Line 4:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Monday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Monday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/rightpic|src=Cambridge-<del class="diffchange diffchange-inline">Postit</del>.jpg}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/rightpic|src=Cambridge-<ins class="diffchange diffchange-inline">16aug</ins>.jpg}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We spent the morning compiling a large set of ideas, each on its own post-it-note. We categorised them and excluded those that were clearly unfeasible. If you like, you can see the [[Team:Cambridge/Ideas |long list]].</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Tuesday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Tuesday==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Theoshttp://2010.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week6&diff=51314&oldid=prevTheos: /* Monday */2010-08-16T11:24:12Z<p><span class="autocomment">Monday</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 11:24, 16 August 2010</td>
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<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/headerbar|colour=#fad72a|title=Notebook: Week 6}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Cambridge/Templates/headerbar|colour=#fad72a|title=Notebook: Week 6}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==Monday==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== Monday==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{:Team:Cambridge/Templates/rightpic|src=Cambridge-Postit.jpg}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We spent the morning compiling a large set of ideas, each on its own post-it-note. We categorised them and excluded those that were clearly unfeasible. If you like, you can see the [[Team:Cambridge/Ideas |long list]].</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Tuesday==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Tuesday==</div></td></tr>
</table>Theos