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Team:Cambridge/Notebook/Week4
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*Wet work | *Wet work | ||
**Transformed competent cells using plasmid phK724 from Jim Slock (thanks!) | **Transformed competent cells using plasmid phK724 from Jim Slock (thanks!) | ||
| - | **Grew up E. coli containing phK555 in 5ml LB broth in rotating | + | **Grew up E. coli containing phK555 in 5ml LB broth in rotating 37°C incubator overnight |
*Dry work | *Dry work | ||
**Finalising sequencing requirements | **Finalising sequencing requirements | ||
| Line 15: | Line 15: | ||
* Wet Work | * Wet Work | ||
** Made competent cells of 4 different hns mutants: W3110 hns93-1, BW25113 Δhns::kan, W3110 hns-205::Tn10, GM230 hns-205::Tn10 | ** Made competent cells of 4 different hns mutants: W3110 hns93-1, BW25113 Δhns::kan, W3110 hns-205::Tn10, GM230 hns-205::Tn10 | ||
| - | ** Now stored in the - | + | ** Now stored in the -80°C freezer for later use |
* Dry Work | * Dry Work | ||
Revision as of 09:32, 4 August 2010
Notebook: Week 4
Monday
- Wet work
- Transformed competent cells using plasmid phK724 from Jim Slock (thanks!)
- Grew up E. coli containing phK555 in 5ml LB broth in rotating 37°C incubator overnight
- Dry work
- Finalising sequencing requirements
- Dismissed PPK
- Decided to include Luciola cruciata
- Further Wiki work
- Finalising sequencing requirements
Tuesday
- Wet Work
- Made competent cells of 4 different hns mutants: W3110 hns93-1, BW25113 Δhns::kan, W3110 hns-205::Tn10, GM230 hns-205::Tn10
- Now stored in the -80°C freezer for later use
- Dry Work
- Organising sequences for synthesis
Wednesdy
Thursday
Friday
Saturday
Sunday
