Team:Cambridge/Notebook/Week3

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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10

Monday

Afternoon

Theo created a plan for an LRE Biobrick, and submitted it to DNA 2.0 for a quote.
Anja, Hannah and Emily worked on text for Sterilin
Peter and Will created primer sequences for first experiment

Tuesday

Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
We organised all the stock in the lab
We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.

Wednesday

Inoculated a broth with Top 10. Making competent cells tomorrow.

Thursday

To do: prepare competent cells

Friday

Preparing order for sythesis
Theo aligned 300 luciferase-sequences to visualise consensus
Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
Anja, Emily and Bill prepared chemically competent cells.

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