Team:Cambridge/Notebook/Week3

From 2010.igem.org

(Difference between revisions)
(Friday)
(Replacing page with ' {{:Team:Cambridge/Templates/HybridBook|num=3}}')
 
(12 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Cambridge/Templates/header}}
 
-
{{:Team:Cambridge/WeekTemplate}}
 
-
== Monday==
 
-
===Afternoon===
 
-
* Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html>, and submitted it to DNA 2.0 for a quote.
 
-
* Anja, Hannah and Emily worked on text for Sterilin
 
-
* Peter and Will created primer sequences for first experiment
 
-
==Tuesday==
+
{{:Team:Cambridge/Templates/HybridBook|num=3}}
-
* Duncan provided several E. coli strains.  We plated out TOP10 to create colonies from which to create competent cells for transformation.
+
-
* We organised all the stock in the lab
+
-
* We met with Dr. Summers to discuss quiescence.  He was very encouraging but have yet to settle IP concerns.  He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
+
-
 
+
-
== Wednesday==
+
-
Inoculated a broth with Top 10.  Making competent cells tomorrow.
+
-
 
+
-
==Thursday==
+
-
To do: prepare competent cells
+
-
 
+
-
== Friday==
+
-
* Preparing order for sythesis
+
-
* Theo aligned 300 luciferase-sequences to visualise consensus
+
-
* Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
+
-
* Anja, Emily and Bill prepared chemically competent cells!
+
-
** By spending an hour in a cold room. It was cold but worth it. We got to drink hot chocolate.
+
-
{{:Team:Cambridge/Templates/footer}}
+

Latest revision as of 22:44, 18 August 2010