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Team:Cambridge/Notebook/Week3
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== Monday== | == Monday== | ||
{{:Team:Cambridge/Templates/rightpic|src=CamNoteWk3_1.jpg}} | {{:Team:Cambridge/Templates/rightpic|src=CamNoteWk3_1.jpg}} | ||
| - | + | Today we had another meeting in the morning to try and distribute jobs. Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html> and submitted it to [https://www.dna20.com/ DNA 2.0] for a quote - we really wanted to get our DNA synthesised as quickly as possible. | |
| - | + | Anja, Hannah and Emily had fun writing a draft article for [http://www.sterilin.co.uk/ Sterilin] describing what iGEM is, what we were hoping to accomplish and what Sterilin products we had been using. Peter and Will also created primer sequences for our first experiment. Quite a productive day! | |
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==Tuesday== | ==Tuesday== | ||
Revision as of 10:06, 10 August 2010
Notebook: Week 3
Week 3: Monday 26th July - Sunday 1st August
Monday
Today we had another meeting in the morning to try and distribute jobs. Theo created a plan for an LRE Biobrick and submitted it to DNA 2.0 for a quote - we really wanted to get our DNA synthesised as quickly as possible. Anja, Hannah and Emily had fun writing a draft article for Sterilin describing what iGEM is, what we were hoping to accomplish and what Sterilin products we had been using. Peter and Will also created primer sequences for our first experiment. Quite a productive day!
Tuesday
- Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
- We organised all the stock in the lab
- We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
Wednesday
Inoculated a broth with Top 10. Making competent cells tomorrow.
Thursday
To do: prepare competent cells
Friday
- Preparing order for sythesis
- Theo aligned 300 luciferase-sequences to visualise consensus
- Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
- Anja, Emily and Bill prepared chemically competent cells!
- By spending an hour in a cold room. It was cold but worth it. We got to drink hot chocolate.
Saturday
- Fernan tested our competent cells for us and they worked!
Sunday
- Anja, Bill, Emily and Theo discussed a final template for the wiki - it is looking pretty.
