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Team:Cambridge/Notebook/Week3
From 2010.igem.org
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| + | * Preparing order for sythesis | ||
| + | * Theo aligned 300 luciferase-sequences to visualise consensus | ||
| + | * Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics | ||
| + | * Anja, Emily and Bill prepared chemically competent cells. | ||
{{:Team:Cambridge/Templates/footer}} | {{:Team:Cambridge/Templates/footer}} | ||
Revision as of 18:15, 30 July 2010
Monday
Afternoon
- Theo created a plan for an LRE Biobrick, and submitted it to DNA 2.0 for a quote.
- Anja, Hannah and Emily worked on text for Sterilin
- Peter and Will created primer sequences for first experiment
Tuesday
- Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
- We organised all the stock in the lab
- We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
Wednesday
Inoculated a broth with Top 10. Making competent cells tomorrow.
Thursday
To do: prepare competent cells
Friday
- Preparing order for sythesis
- Theo aligned 300 luciferase-sequences to visualise consensus
- Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
- Anja, Emily and Bill prepared chemically competent cells.
