Team:Cambridge/Notebook/Week2

From 2010.igem.org

(Difference between revisions)
m (header)
(Replacing page with ' {{:Team:Cambridge/Templates/HybridBook|num=2}}')
 
(28 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Cambridge/Templates/headerMinimalprototype}}
 
-
{{:Team:Cambridge/Templates/headerbar|colour=#fad72a|title=Notebook: Week 2}}
 
-
== Monday==
+
{{:Team:Cambridge/Templates/HybridBook|num=2}}
-
Did further research into the enzymes required for luciferase recovery.
+
-
* Presented proposals to advisors
+
-
** Modelled RNA to create aptazyme based system using trial of CLC RNA Workbench
+
-
** Made presentation for Newcastle
+
-
** Set up and tested low light camera
+
-
 
+
-
== Tuesday==
+
-
[[Image:CambridgeTicket.jpg|200px|right|frame]]
+
-
* 06.50 - Train to Newcastle for UK iGEM get-togther
+
-
 
+
-
== Wednesday==
+
-
 
+
-
 
+
-
== Thursday==
+
-
*11am - Health and Safety talk by Barbara
+
-
*Working lunch at the Waffle Company. Summary of discussion:
+
-
**'''To do:'''
+
-
***Ordering
+
-
***Getting in touch with MIT (turning off lights)
+
-
***T-Shirts
+
-
***Project Plan (Gantt Chart etc)
+
-
**'''To do long-term:'''
+
-
***Modelling
+
-
***Firefly Bioluminescence
+
-
***Bacterial Bioluminescence
+
-
***Human Practices
+
-
***Quiescence
+
-
**'''Individual roles:'''
+
-
***Outline of experiments, protocols - Anja, Ben, Peter (especially controls)
+
-
***Modelling - Paul, Bill, Emily
+
-
***COSHH forms - Bill
+
-
***LRE biobrick - Theo
+
-
***Researching Bacterial Lux operon - Will, Hannah
+
-
 
+
-
== Friday==
+
-
Meeting with Laura Rowe in the Biotechnology Dept. at 3pm:
+
-
*'''Brightness measurements''' are integrated over time, so when comparing them make sure they are integrated over a long time (otherwise you are only measuring how quickly luminescence occurs, not how bright).
+
-
*'''Different colours''':
+
-
**Could use fluorophores instead of fluorescent proteins but need to ensure they attach to protein (could be a project in itself). Requires resonance energy transfer.
+
-
**Using mutant luciferases probably easier to implement.
+
-
*'''Expression in ''E. coli''''': inclusion bodies could be a problem when trying to over-express genes. To test: break open cells and centrifuge twice (at a higher speed second time around), if pellets are formed these are probably inclusion bodies. Could then dissolve these with solvent and test for bioluminescence from proteins within inclusion bodies.
+
-
*'''Relative light units''' are used because different camera properties, distances etc. all mean that photons/sec are relative. Can use a source for calibration - tritium standards are used (e.g. MGM instruments).
+
-
 
+
-
Paper from Duncan about mutant bacterial strain with very bright luminescence to be investigated further.
+
-
 
+
-
== Saturday==
+
-
== Sunday==
+
-
<html>
+
-
</div>
+
-
</html>
+
-
 
+
-
{{:Team:Cambridge/Templates/footerMinimal}}
+

Latest revision as of 22:43, 18 August 2010