Team:Cambridge/Notebook/Week2

From 2010.igem.org

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<div style="width:730px; background:#fad72a;" class="secheader">Week 2</div>
 
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== Monday==
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Did further research into the enzymes required for luciferase recovery.
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* Presented proposals to advisors
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** Modelled RNA to create aptazyme based system using trial of CLC RNA Workbench
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** Made presentation for Newcastle
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** Set up and tested low light camera
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== Tuesday==
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[[Image:CambridgeTicket.jpg|200px|right|frame]]
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* 06.50 - Train to Newcastle for UK iGEM get-togther
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== Wednesday==
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== Thursday==
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*11am - Health and Safety talk by Barbara
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*Working lunch at the Waffle Company. Summary of discussion:
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**'''To do:'''
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***Ordering
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***Getting in touch with MIT (turning off lights)
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***T-Shirts
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***Project Plan (Gantt Chart etc)
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**'''To do long-term:'''
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***Modelling
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***Firefly Bioluminescence
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***Bacterial Bioluminescence
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***Human Practices
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***Quiescence
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**'''Individual roles:'''
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***Outline of experiments, protocols - Anja, Ben, Peter (especially controls)
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***Modelling - Paul, Bill, Emily
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***COSHH forms - Bill
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***LRE biobrick - Theo
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***Researching Bacterial Lux operon - Will, Hannah
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== Friday==
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Meeting with Laura Rowe in the Biotechnology Dept. at 3pm:
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*'''Brightness measurements''' are integrated over time, so when comparing them make sure they are integrated over a long time (otherwise you are only measuring how quickly luminescence occurs, not how bright).
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*'''Different colours''':
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**Could use fluorophores instead of fluorescent proteins but need to ensure they attach to protein (could be a project in itself). Requires resonance energy transfer.
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**Using mutant luciferases probably easier to implement.
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*'''Expression in ''E. coli''''': inclusion bodies could be a problem when trying to over-express genes. To test: break open cells and centrifuge twice (at a higher speed second time around), if pellets are formed these are probably inclusion bodies. Could then dissolve these with solvent and test for bioluminescence from proteins within inclusion bodies.
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*'''Relative light units''' are used because different camera properties, distances etc. all mean that photons/sec are relative. Can use a source for calibration - tritium standards are used (e.g. MGM instruments).
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Paper from Duncan about mutant bacterial strain with very bright luminescence to be investigated further.
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== Saturday==
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== Sunday==
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Latest revision as of 22:43, 18 August 2010