Team:Cambridge/Notebook/8

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Contents

Monday

Cambridge-Transilluminator.jpg

Anja repeated lux operon extraction with pJS-555 instead of pSK-555 in the hope of getting better results.

Tuesday

Sadly no light from the cells transformed with the extracted lux operon.

Wednesday

Theo attempted to test induction of pBAD in the BMG Labtech plate reader

Thursday

Cambridge-Merged.png

We came in in the morning and viewed the results from the plate reader experiment that had been running overnight. The bacterial lux results were disappointing. Our positive control was emitting (blue) light. (See photo on the right), but the other cells which had been incubated with arabinose in the hope of inducing them had not emitted light.

But the most interesting results came from the cells expressing firefly luciferase (BBa_I712019) under a consitutive promoter / RBS (BBa_J13002. These showed low light output for 13 hours and then a rapid increase in luminescence, to a level which was sustained until the evening. We left the cells in the reader overnight to continue reading.

Friday

Saturday

Sunday

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