Team:Cambridge/LabBook/Week11

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(New page: <div align="center">Week 11: Monday 20th September - Sunday 26th September</div> ==Monday== ===107. Expt: Biobrick assembly of pBAD+Luc (PP + LC) and (Emily)=== *Plasmids already extracted...)
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Revision as of 14:23, 1 October 2010

Week 11: Monday 20th September - Sunday 26th September

Monday

107. Expt: Biobrick assembly of pBAD+Luc (PP + LC) and (Emily)

  • Plasmids already extracted
  • Restriction digest following protocol on p88 using these quantities (in µl):
pBAD PP Luc (1) PP Luc (2) LC Luc (1)
Nuclease-free H20 14 14 14 14
10x FD Buffer 2 2 2 2
Plasmid DNA 2 2 2 2
FD EcoRI 1 1 1 1
FD SpeI 1 0 0 0
FD XbaI 0 1 1 1


µl°C