Team:Cambridge/Gibson/Protocol

From 2010.igem.org

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(Step 3: PCR)
(Step 3: PCR)
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<div align="center">
<div align="center">
<table class="vistable" padding="0" cellspacing="0">  
<table class="vistable" padding="0" cellspacing="0">  
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<tr>
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<td>Step
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</td><td>Temp
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</td><td>Time
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</td></tr>
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<tr>
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<td>1:Initial Melting
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</td><td>98°C
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</td><td>30s
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</td></tr>
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<tr>
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<td>2:Melting
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</td><td>98°C
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</td><td>10s
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</td></tr>
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<tr>
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<td>3:Annealing
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</td><td>T<sub>m</sub>°C
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</td><td>15s
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</td></tr>
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<tr>
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<td>4:Elongation
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</td><td>72
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</td><td>45s per kb DNA
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</td></tr>
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<tr>
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<td>5:GoTo step 2
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</td><td>
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</td><td>30 times
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</td></tr>
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<tr>
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<td>6:Final Elongation
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</td><td>72°C
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</td><td>7m30
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</td></tr>
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<tr>
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<td>7:Final Hold
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</td><td>4°C
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</td><td>∞
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</td></tr>
   
   
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<tr>
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<td><b>Assembly Method</b>
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</td><td><b>Number of steps to ligate N pieces of DNA</b>
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</td></tr>
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</table>  
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<tr>
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<td><a href="http://partsregistry.org/Assembly:Standard_assembly" class="external text" title="http://partsregistry.org/Assembly:Standard_assembly" rel="nofollow">Standard Assembly</a>
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</td><td><a href="/Image:Steps-N.png" class="image" title="Image:Steps-N.png"><img alt="Image:Steps-N.png" src="/wiki/images/c/cc/Steps-N.png"  border="0" /></a>
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</td></tr>
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<tr>
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<td><a href="http://partsregistry.org/Assembly:Rolling_assembly" class="external text" title="http://partsregistry.org/Assembly:Rolling_assembly" rel="nofollow">Parallel Assembly</a>
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</td><td><a href="/Image:Steps-Log2.png" class="image" title="Image:Steps-Log2.png"><img alt="Image:Steps-Log2.png" src="/wiki/images/6/6f/Steps-Log2.png" width="138" height="21" border="0" /></a> (rounded up)
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</td></tr>
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<tr>
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<td><a href="http://2010.igem.org/Team:Cambridge/Gibson/Mechanism" class="external text" title="http://2010.igem.org/Team:Cambridge/Gibson/Mechanism" rel="nofollow">Gibson Assembly</a>
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</td><td><a href="/Image:Steps1.png" class="image" title="Image:Steps1.png"><img alt="Image:Steps1.png" src="/wiki/images/1/10/Steps1.png" width="83" height="18" border="0" /></a>
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</td></tr></table>  
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</div>
</div>
</html>
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Revision as of 19:35, 24 October 2010