Team:Cambridge/Gibson/Protocol

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(Step 1: Design Primers)
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==Step 1: Design Primers==
==Step 1: Design Primers==
{{:Team:Cambridge/Templates/RightImage|image=Cambridge-oligoface.jpg|caption=Designing Oligos Old-School}}
{{:Team:Cambridge/Templates/RightImage|image=Cambridge-oligoface.jpg|caption=Designing Oligos Old-School}}
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{{:Team:Cambridge/Templates/RightImage|image=Gibthon.png|caption=New and improved Gibthon oligo design}}
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*If you wish to ligate two pieces of DNA using Gibson they must be altered so as to have 40bp of overlap at the point of ligation.
*If you wish to ligate two pieces of DNA using Gibson they must be altered so as to have 40bp of overlap at the point of ligation.
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==Step 2: Order Primers==
==Step 2: Order Primers==
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{{:Team:Cambridge/Templates/RightImage|image=Gibthon.png|caption=New and improved Gibthon oligo design}}
*This step can take a while, so Gibson Assembly requires some planning ahead
*This step can take a while, so Gibson Assembly requires some planning ahead

Revision as of 20:32, 23 October 2010