Team:Cambridge/Gibson/Protocol

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==Step 1: Design Primers==
==Step 1: Design Primers==
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{{:Team:Cambridge/Templates/RightImage|image=Cambridge-oligoface.jpg|caption=Designing Oligos Old-School}}
*If you wish to ligate two pieces of DNA using Gibson they must be altered so as to have 40bp of overlap at the point of ligation.
*If you wish to ligate two pieces of DNA using Gibson they must be altered so as to have 40bp of overlap at the point of ligation.

Revision as of 20:26, 23 October 2010