Team:Cambridge/Gibson/Protocol

From 2010.igem.org

(Difference between revisions)
(Step 3: PCR)
Line 28: Line 28:
|Time
|Time
|-
|-
-
|1
+
|1:Initial Melting
|98°C
|98°C
|30s
|30s
|-
|-
-
|T<sub>m</sub>
+
|2:Melting
-
|
+
|98°C
-
|
+
|10s
|-
|-
 +
|3:Annealing
 +
|T<sub>m</sub>°C
 +
|15s
 +
|-
 +
|4:Elongation
 +
|72
 +
|45s/kb
 +
|-
 +
|5:GoTo step 2
|
|
-
|
+
|30 times
-
|
+
|-
 +
|6:Final Elongation
 +
|72°C
 +
|7m30
 +
|-
 +
|7:Final Hold
 +
|4°C
 +
|∞
|}
|}

Revision as of 20:14, 23 October 2010