Team:Cambridge/Gibson/Protocol

From 2010.igem.org

(Difference between revisions)
Line 17: Line 17:
==Step 3: PCR ==
==Step 3: PCR ==
 +
 +
*PCR is a dark art, but we have found that these general principles have served us well over the summer.
 +
 +
**98°C 30s
 +
 +
{|class="wikitable"
 +
|-
 +
|Step
 +
|Temp
 +
|Time
 +
|-
 +
|1
 +
|98°C
 +
|30s
 +
|-
 +
|T<sub>m</sub>
 +
|
 +
|
 +
|-
 +
|
 +
|
 +
|
 +
|}
==Step 4: Gibson Assembly==
==Step 4: Gibson Assembly==
Line 38: Line 61:
|}
|}
 +
 +
==Step 5: Transformation==
{{:Team:Cambridge/Templates/footer}}
{{:Team:Cambridge/Templates/footer}}

Revision as of 16:16, 23 October 2010