Team:Cambridge/Gibson/Protocol

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(Difference between revisions)
(Step 1: Design Primers)
(Step 1: Design Primers)
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If you wish to ligate two pieces of DNA using Gibson they must be altered so as to have 40bp of overlap at the point of ligation.
If you wish to ligate two pieces of DNA using Gibson they must be altered so as to have 40bp of overlap at the point of ligation.
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The standard way to do this is with PCR with specialised primers (see [[Team:Cambridge/Gibson/Mechanism |mechanism]]. We have designed a tool to help you do this: [http://www.gibthon.org Gibthon]
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The standard way to do this is with PCR with specialised primers (see [[Team:Cambridge/Gibson/Mechanism |mechanism]]). We have designed a tool to help you do this: [http://www.gibthon.org Gibthon]
==Step 2: Order Primers==
==Step 2: Order Primers==

Revision as of 20:25, 26 October 2010