Team:Cambridge/Gibson/Protocol

From 2010.igem.org

(Difference between revisions)
(Step 4: Gibson Assembly)
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==Step 4: Gibson Assembly==
==Step 4: Gibson Assembly==
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1)Prepare [[Team:Cambridge/Gibson/MasterMix | Gibson Master Mix]]
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1) Prepare [[Team:Cambridge/Gibson/MasterMix | Gibson Master Mix]]
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2)Add DNA to be ligated and Master Mix in volumetric ratio 1:3
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2) Add DNA to be ligated and Master Mix in volumetric ratio 1:3
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3)Incubate for 1 hour at 50°C
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3) Incubate for 1 hour at 50°C

Revision as of 19:31, 24 October 2010