Team:Cambridge/Gibson/Protocol

From 2010.igem.org

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{{:Team:Cambridge/Templates/headerMinimalprototype}}
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{{:Team:Cambridge/Templates/headerbar|colour=#fb5c2b|title=Gibson Assembly: Protocols}}
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{{:Team:Cambridge/Templates/headerbar|colour=#fb5c2b|title=Gibson Assembly: Protocol}}
The formal paper in nature describing Gibson Assembly can be found [http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html here].
The formal paper in nature describing Gibson Assembly can be found [http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html here].
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==Step 2: Order Primers==
==Step 2: Order Primers==
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*This step can take a while, so Gibson Assembly requires some planning ahead
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This step can take a while, so Gibson Assembly requires some planning ahead
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==Step 4: Gibson Assembly==
==Step 4: Gibson Assembly==
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*Prepare [[Team:Cambridge/Gibson/MasterMix | Gibson Master Mix]]
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1)Prepare [[Team:Cambridge/Gibson/MasterMix | Gibson Master Mix]]
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*Add DNA to be ligated and Master Mix in volumetric ratio 1:3
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*Incubate for 1 hour at 50°C
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2)Add DNA to be ligated and Master Mix in volumetric ratio 1:3
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3)Incubate for 1 hour at 50°C

Revision as of 19:31, 24 October 2010