Team:Cambridge/Gibson/Mechanism

From 2010.igem.org

(Difference between revisions)
(Creating overlapping DNA sequences)
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<html><div style="text-align:center"><img src="http://2010.igem.org/wiki/images/a/a3/Cambridge-Gib1.png" style="border:1px solid gray; margin-top:20px; margin-bottom:20px;"></div></html>
<html><div style="text-align:center"><img src="http://2010.igem.org/wiki/images/a/a3/Cambridge-Gib1.png" style="border:1px solid gray; margin-top:20px; margin-bottom:20px;"></div></html>
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Note:
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*The end of sequence A with the extension now looks like
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.....endA+begB
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*The beginning of sequence B looks like
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endA+begB.......
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*This results in there now being a total overlap of 40bp between the two fragments, enough to perform Gibson assembly.
== Gibson Assembly==
== Gibson Assembly==

Revision as of 20:28, 27 October 2010