Team:Cambridge/Gibson/Mechanism

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By using two such primers we can add 20 bp of sequence A to sequence B and 20 bp of sequence B to sequence A.  We are then ready to use Gibson Assembly.  The Cambridge team have developed [http://www.gibthon.org/ Gibthon] to help you design primers for Gibson Assembly.  The tool allows you to put in two sequences and choose 20bp of each to get a 40bp primer; it then analyses the melting temperature and secondary structure of this primer.
By using two such primers we can add 20 bp of sequence A to sequence B and 20 bp of sequence B to sequence A.  We are then ready to use Gibson Assembly.  The Cambridge team have developed [http://www.gibthon.org/ Gibthon] to help you design primers for Gibson Assembly.  The tool allows you to put in two sequences and choose 20bp of each to get a 40bp primer; it then analyses the melting temperature and secondary structure of this primer.
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== Gibson Assembly==
== Gibson Assembly==

Revision as of 03:11, 26 October 2010