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Team:Cambridge/Gibson/MasterMix
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Willh (Talk | contribs)
(New page: {|class="wikitable" |- | |Volume/µl |- |Taq ligase (40u/µl) |50 |- |5x isothermal buffer |100 |- |T5 exonuclease (1u/µl) |2 |- |Phusion polymerase (2u/µl) |6.25 |- |Nuclease-free wate...)
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(New page: {|class="wikitable" |- | |Volume/µl |- |Taq ligase (40u/µl) |50 |- |5x isothermal buffer |100 |- |T5 exonuclease (1u/µl) |2 |- |Phusion polymerase (2u/µl) |6.25 |- |Nuclease-free wate...)
Newer edit →
Revision as of 13:18, 30 September 2010
| Volume/µl | |
| Taq ligase (40u/µl) | 50 |
| 5x isothermal buffer | 100 |
| T5 exonuclease (1u/µl) | 2 |
| Phusion polymerase (2u/µl) | 6.25 |
| Nuclease-free water | 216.75 |
| =375 |
Master Mix is 1.33x concentrated
