Team:Cambridge/Gibson/Introduction

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Gibson Assembly is a cutting-edge DNA ligation technique developed by Dan Gibson at JCVI in 2009 [http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1318.html].  
Gibson Assembly is a cutting-edge DNA ligation technique developed by Dan Gibson at JCVI in 2009 [http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1318.html].  
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It is an enzymatic assembly technique for ligating two or more sequences of DNA when they have overlapping end sequences at their joining point (~40bp). These overlapping regions can be easily added to the ends of any length of DNA by using PCR with primers which have added "flaps". Thus PCR followed by Gibson allows you to join any two blunt ended pieces of DNA.
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It uses enzymes to ligate two or more sequences of DNA when they have overlapping end sequences at their joining point (~40bp). These overlapping regions can be easily added to the ends of any length of DNA by using PCR with primers which have added "flaps". Thus PCR followed by Gibson allows you to join any two blunt ended pieces of DNA.
== Advantages ==
== Advantages ==

Revision as of 19:00, 24 October 2010