Team:Cambridge/Gibson/Introduction

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{{:Team:Cambridge/Templates/headerbar|colour=#fb5c2b|title=Gibson Assembly: Introduction}}
{{:Team:Cambridge/Templates/headerbar|colour=#fb5c2b|title=Gibson Assembly: Introduction}}
{{:Team:Cambridge/Templates/RightImage|image=GibsonAssembly.jpg|caption=Gibson Assembly joins any two lengths of DNA which have overlapping end regions}}
{{:Team:Cambridge/Templates/RightImage|image=GibsonAssembly.jpg|caption=Gibson Assembly joins any two lengths of DNA which have overlapping end regions}}
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Gibson Assembly is a cutting-edge DNA ligation technique developed by Dan Gibson at JCVI in 2009 [http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1318.html].  
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'''Gibson Assembly''' is a cutting-edge DNA ligation technique developed by Dan Gibson at JCVI in 2009 [http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1318.html].  
It uses three enzymes to ligate two or more sequences of DNA when they have overlapping end sequences at their joining point (~40bp). These overlapping regions can be easily added to the ends of any length of DNA by using PCR with primers which have added "flaps". Thus PCR followed by Gibson allows you to join any two blunt ended pieces of DNA.
It uses three enzymes to ligate two or more sequences of DNA when they have overlapping end sequences at their joining point (~40bp). These overlapping regions can be easily added to the ends of any length of DNA by using PCR with primers which have added "flaps". Thus PCR followed by Gibson allows you to join any two blunt ended pieces of DNA.
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3) Any two blunt ended pieces of DNA can be joined, and DNA can be taken from any source which is accessible to PCR (for example, an organism's '''genome''')
3) Any two blunt ended pieces of DNA can be joined, and DNA can be taken from any source which is accessible to PCR (for example, an organism's '''genome''')
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4) Although it is roughly the same speed as [http://partsregistry.org/Help:BioBrick_Assembly standard BioBrick assembly] when ligating two fragments, Gibson is perfect for doing multi part systems, since it takes the same amount of time to ligate n pieces of DNA together as it does for two pieces.
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4) Although it is roughly the same speed as [http://partsregistry.org/Help:BioBrick_Assembly standard BioBrick assembly] when ligating two fragments, Gibson is perfect for '''assembling multi part systems''', since it takes the same amount of time to ligate n pieces of DNA together as it does for two pieces:
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Latest revision as of 20:47, 27 October 2010