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Team:Cambridge/Gibson/Introduction
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== Advantages == | == Advantages == | ||
| - | * No scar created - useful '''fusion proteins''' and '''adding an RBS''', where scars can be problematic. | + | * No scar created - useful for '''fusion proteins''' and '''adding an RBS''', where scars can be problematic. |
| - | * Can | + | * Can re-ligate linear DNA into a circle - useful for '''site-directed mutagenesis''' |
| - | * Faster than Biobrick assembly, and works with less | + | * Faster than Biobrick assembly, and works with less original. |
{{:Team:Cambridge/Templates/footer}} | {{:Team:Cambridge/Templates/footer}} | ||
Revision as of 15:05, 18 September 2010
Gibson Assembly: Introduction
Gibson Assembly is a technique for assembling DNA with short (c. 40 bp) overlapping sequences together. Since these overlapping regions can be easily added by PCR with primers which have added "flaps", any DNA sequences can be joined by this mechanism.
Advantages
- No scar created - useful for fusion proteins and adding an RBS, where scars can be problematic.
- Can re-ligate linear DNA into a circle - useful for site-directed mutagenesis
- Faster than Biobrick assembly, and works with less original.
