Team:Cambridge/Gibson/Introduction

From 2010.igem.org

(Difference between revisions)
(Gibson Assembly)
(Advantages)
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2) Can re-ligate linear DNA into a circle - useful for '''site-directed mutagenesis'''
2) Can re-ligate linear DNA into a circle - useful for '''site-directed mutagenesis'''
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3) Any two blunt ended pieces of DNA can be joined, and DNA can be taken from any source which is acessible to PCR (for example, an organism's '''genome''')
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3) Any two blunt ended pieces of DNA can be joined, and DNA can be taken from any source which is accessible to PCR (for example, an organism's '''genome''')
4) Although it is roughly the same speed as [http://partsregistry.org/Help:BioBrick_Assembly standard BioBrick assembly] when ligating two fragments, Gibson is perfect for doing multi part systems, since it takes the same amount of time to ligate n pieces of DNA together as it does for two pieces.
4) Although it is roughly the same speed as [http://partsregistry.org/Help:BioBrick_Assembly standard BioBrick assembly] when ligating two fragments, Gibson is perfect for doing multi part systems, since it takes the same amount of time to ligate n pieces of DNA together as it does for two pieces.

Revision as of 19:16, 24 October 2010