Team:Cambridge/Gibson/Introduction

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Gibson Assembly is a cutting-edge DNA ligation technique developed by Dan Gibson at JCVI in 2009 [http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1318.html].  
Gibson Assembly is a cutting-edge DNA ligation technique developed by Dan Gibson at JCVI in 2009 [http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1318.html].  
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It is an enzymatic assembly technique for ligating lengths of DNA with short (c. 40 bp) overlapping end sections. These overlapping regions can be easily added to the end of a section of DNA by using PCR with primers which have added "flaps". This means that DNA sequences can be joined by this mechanism.
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It is an enzymatic assembly technique for ligating sequences of DNA with short (c. 40 bp) overlapping end sections. These overlapping regions can be easily added to the ends of a length of DNA by using PCR with primers which have added "flaps".
== Advantages ==
== Advantages ==

Revision as of 16:06, 23 October 2010