Team:Cambridge/BioluminescenceLinks
From 2010.igem.org
(Difference between revisions)
(→Ben's section) |
EmilyKnott (Talk | contribs) (→Emily's Section) |
||
Line 2: | Line 2: | ||
=Bioluminescence= | =Bioluminescence= | ||
==Emily's Section== | ==Emily's Section== | ||
- | *Metridia luciferase | + | *Spectra: |
+ | **Spectral Emissions of different species - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Different Species Spectra] | ||
+ | **Fluorescent Protein Spectra - [http://jcs.biologists.org/cgi/reprint/114/5/837 Spectra] | ||
+ | *Metridia: (luciferase requires CBP Protein) | ||
**Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048 | **Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048 | ||
**CBP link - http://www.ncbi.nlm.nih.gov/pubmed/18264586 | **CBP link - http://www.ncbi.nlm.nih.gov/pubmed/18264586 | ||
**CBP amino acid sequence http://www.rsc.org/ej/PP/2008/b713109g.pdf | **CBP amino acid sequence http://www.rsc.org/ej/PP/2008/b713109g.pdf | ||
+ | *Firefly: | ||
**Oxyluciferin reduction - http://www.ncbi.nlm.nih.gov/pubmed/19872330 | **Oxyluciferin reduction - http://www.ncbi.nlm.nih.gov/pubmed/19872330 | ||
**Luciferin Regenerating Enzyme (LRE) - [http://www.ncbi.nlm.nih.gov/pubmed/11457857 LRE] (in ''Photinus pyralis'') | **Luciferin Regenerating Enzyme (LRE) - [http://www.ncbi.nlm.nih.gov/pubmed/11457857 LRE] (in ''Photinus pyralis'') | ||
- | |||
**Firefly luciferin reaction - http://pubs.acs.org/doi/pdf/10.1021/ja00529a051 | **Firefly luciferin reaction - http://pubs.acs.org/doi/pdf/10.1021/ja00529a051 | ||
**Light Emission Quanta - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WB5-4DXBGFJ-D5-1&_cdi=6701&_user=1495569&_pii=0003986160902083&_orig=search&_coverDate=05%2F31%2F1960&_sk=999119998&view=c&wchp=dGLbVzz-zSkzV&md5=f1b9d348749d1304c05f85d9290e3b90&ie=/sdarticle.pdf Spectral Emission and Quantum Yield of Firefly Bioluminescence] | **Light Emission Quanta - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WB5-4DXBGFJ-D5-1&_cdi=6701&_user=1495569&_pii=0003986160902083&_orig=search&_coverDate=05%2F31%2F1960&_sk=999119998&view=c&wchp=dGLbVzz-zSkzV&md5=f1b9d348749d1304c05f85d9290e3b90&ie=/sdarticle.pdf Spectral Emission and Quantum Yield of Firefly Bioluminescence] | ||
***One light quantum emitted for every luciferin molecule oxidized | ***One light quantum emitted for every luciferin molecule oxidized | ||
**CoA stimulation - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B73G9-47G3TGV-1S9-1&_cdi=11521&_user=1495569&_pii=0006300258903810&_orig=search&_coverDate=12%2F31%2F1958&_sk=999729999&view=c&wchp=dGLbVzb-zSkzV&md5=4420b38ef24aba8ca23e0b8ce2b3a953&ie=/sdarticle.pdf CoA in Luminescence] | **CoA stimulation - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B73G9-47G3TGV-1S9-1&_cdi=11521&_user=1495569&_pii=0006300258903810&_orig=search&_coverDate=12%2F31%2F1958&_sk=999729999&view=c&wchp=dGLbVzb-zSkzV&md5=4420b38ef24aba8ca23e0b8ce2b3a953&ie=/sdarticle.pdf CoA in Luminescence] | ||
+ | *Aequorin: | ||
+ | **Regeneration of Aequorin in ''Aequorea Aequorea'' jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf | ||
+ | *Bacteria: | ||
**Bacterial luciferase reaction - [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC357244/?page=1 Bacterial Luciferin] | **Bacterial luciferase reaction - [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC357244/?page=1 Bacterial Luciferin] | ||
**Beetle luciferin - [http://www3.interscience.wiley.com/cgi-bin/fulltext/107615738/PDFSTART Putative Beetle Luciferin Synthesis] | **Beetle luciferin - [http://www3.interscience.wiley.com/cgi-bin/fulltext/107615738/PDFSTART Putative Beetle Luciferin Synthesis] | ||
**V. Fischeri Plasmid - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WSN-4C7WG76-M-1&_cdi=7051&_user=1495569&_pii=0092867483900636&_orig=search&_coverDate=03%2F31%2F1983&_sk=999679996&view=c&wchp=dGLzVlz-zSkWb&md5=97c3a918d59cbd913da5c00b6805deb9&ie=/sdarticle.pdf V. Fischeri Plasmid] | **V. Fischeri Plasmid - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WSN-4C7WG76-M-1&_cdi=7051&_user=1495569&_pii=0092867483900636&_orig=search&_coverDate=03%2F31%2F1983&_sk=999679996&view=c&wchp=dGLzVlz-zSkWb&md5=97c3a918d59cbd913da5c00b6805deb9&ie=/sdarticle.pdf V. Fischeri Plasmid] | ||
**KCal/Mol luciferin in Lightning Bugs - [http://www.springerlink.com/content/w184700gj8767ww7/fulltext.pdf Luciferin KCal/Mol] | **KCal/Mol luciferin in Lightning Bugs - [http://www.springerlink.com/content/w184700gj8767ww7/fulltext.pdf Luciferin KCal/Mol] | ||
- | |||
**Photinus Pyralis (firefly) spectrum, 562nm - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Reference] | **Photinus Pyralis (firefly) spectrum, 562nm - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Reference] | ||
- | |||
**Different Coloured Firefly Luciferases - [http://peds.oxfordjournals.org/cgi/reprint/18/12/581 Colours] | **Different Coloured Firefly Luciferases - [http://peds.oxfordjournals.org/cgi/reprint/18/12/581 Colours] | ||
**Small amount of blue-shift - [http://pubs.acs.org/doi/pdfplus/10.1021/bi7015052 Blue Shift] | **Small amount of blue-shift - [http://pubs.acs.org/doi/pdfplus/10.1021/bi7015052 Blue Shift] | ||
+ | *Gaussia Princeps (copepod): | ||
+ | **GLuc - [http://aem.asm.org/cgi/content/abstract/71/7/3427 GLuc] | ||
+ | **100 Times Brighter than P. Pyrilus!! - [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth979.html So bright] | ||
==Theo's section== | ==Theo's section== |
Revision as of 15:35, 16 July 2010
Bioluminescence
Emily's Section
- Spectra:
- Spectral Emissions of different species - Different Species Spectra
- Fluorescent Protein Spectra - Spectra
- Metridia: (luciferase requires CBP Protein)
- Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048
- CBP link - http://www.ncbi.nlm.nih.gov/pubmed/18264586
- CBP amino acid sequence http://www.rsc.org/ej/PP/2008/b713109g.pdf
- Firefly:
- Oxyluciferin reduction - http://www.ncbi.nlm.nih.gov/pubmed/19872330
- Luciferin Regenerating Enzyme (LRE) - LRE (in Photinus pyralis)
- Firefly luciferin reaction - http://pubs.acs.org/doi/pdf/10.1021/ja00529a051
- Light Emission Quanta - Spectral Emission and Quantum Yield of Firefly Bioluminescence
- One light quantum emitted for every luciferin molecule oxidized
- CoA stimulation - CoA in Luminescence
- Aequorin:
- Regeneration of Aequorin in Aequorea Aequorea jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf
- Bacteria:
- Bacterial luciferase reaction - Bacterial Luciferin
- Beetle luciferin - Putative Beetle Luciferin Synthesis
- V. Fischeri Plasmid - V. Fischeri Plasmid
- KCal/Mol luciferin in Lightning Bugs - Luciferin KCal/Mol
- Photinus Pyralis (firefly) spectrum, 562nm - Reference
- Different Coloured Firefly Luciferases - Colours
- Small amount of blue-shift - Blue Shift
- Gaussia Princeps (copepod):
Theo's section
- Self contained part for Photorhabdus luminescens lux operon: http://partsregistry.org/Part:BBa_J70008:Design
- Firefly diagram from Emily's paper:
- Fireflow luciferin normally emits at 560nm but at higher temperatures at 630nm
- http://pubs.acs.org/doi/pdf/10.1021/bi00702a015 Kinetics
- Mg2+ required
- A nice background summary: http://irishmarinelife.com/bioluminescence-symbioses/
- The guy who made the biobrick's logbook: http://openwetware.org/wiki/User:Spenser_Skates/Notebook/pTKX_luciferase_biobrick/2008/04/14
Vibrio
- luxC = acyl coA reductase
Firefly
- The awesome paper http://www.springerlink.com/content/67w1q80435537747/fulltext.pdf
Bill's section
- Conversion of normal luciferin into firefly luciferin
- Don't use luciferinol, it r teh suckage
- Glowing fish what?
Will's section
Anja's section
- Paper on mutant luciferase with increased activity
- Aequorin wavelength= 469 nm
- Low leakage rate
- Does not disrupt cell functions/ devt.
- Requires continual addition of luciferin
- Dr Laura Rowe's Paper on Bioluminescent Proteins
- Paper on Biosynthesis of Firefly D-Luciferin
- Paper on L-Tryptophan in Biosynthesis of Sea Firefly Luciferase
Peter's section
Ben's section
- Flashlight fish symbiont luciferase genes have been sequenced but cannot be sucessfully expressed in E. Coli
- Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/
- The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html
- Firefly luciferin biosynthesis appears not to be known. Vibrio Ficheri Lux operon is known. Would VF luciferin work with other luciferases? http://www3.interscience.wiley.com/cgi-bin/fulltext/89014035/PDFSTART
Paul's section
- Precursor to luciferin: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=261858|ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
- Construction of a new firefly bioluminescence system using l-luciferin as substrate
- stuff to do with ATP in the cycle
- Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase
- Biobrick for the Firefly luciferase for Slovenia '07: http://partsregistry.org/Part:BBa_I712019:Design